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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||IP, IF, IHC, ICC, WB|
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Anti-DYNLL1/Pin Rabbit Monoclonal Antibody
Boster Bio Anti-DYNLL1/Pin Rabbit Monoclonal Antibody catalog # M03454. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DYNLL1/Pin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03454)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human DYNLL1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M03454 is reactive to DYNLL1 in Human, Mouse, Rat
M03454 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
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Western blot analysis of DYNLL1 expression in HeLa cell lysate (M03454).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DYNLL1 monoclonal antibody (Catalog # M03454) overnight at 4 °C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DYNLL1
Gene/Protein Information For DYNLL1 (Source: Uniprot.Org, NCBI)
Dynein light chain 1, cytoplasmic
dynein light chain family
cytoplasmic dynein light polypeptide; DLC1; DLC1DNCLC1; DLC8; DLC8MGC126137; DNCL1; DNCL1MGC126138; DNCLC1; dynein light chain 1, cytoplasmic; Dynein light chain LC8-type 1; dynein, cytoplasmic, light polypeptide 1; dynein, light chain, LC8-type 1,8 kDa dynein light chain; DYNLL1; hdlc1; LC8; LC8a; PIN; PINLC8a; Protein inhibitor of neuronal nitric oxide synthase DYNLL1 DLC1, DLC8, DNCL1, DNCLC1, LC8, LC8a, PIN, hdlc1 dynein light chain LC8-type 1 dynein light chain 1, cytoplasmic|8 kDa dynein light chain|cytoplasmic dynein light polypeptide|dynein, cytoplasmic, light polypeptide 1|protein inhibitor of neuronal nitric oxide synthase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on DYNLL1, check out the DYNLL1 Infographic
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In this infographic you will see the following information for DYNLL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for M03454
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