Anti-DYRK1A Antibody Picoband™

Product Name

Anti-DYRK1A Antibody Picoband™

View all DYRK1A Antibodies

SKU/Catalog Number

A00878-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DYRK1A Antibody Picoband™ catalog # A00878-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DYRK1A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00878-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human DYRK1A recombinant protein (Position: H38-M269).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00878-1 is reactive to DYRK1A in Human, Rat

Applications

A00878-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

100 kDa

Calculated molecular weight

85.584kDa

Background of DYRK1A

Dual specificity tyrosine-phosphorylation-regulated kinase 1A is an enzyme that in humans is encoded by the DYRK1A gene. This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of DYRK1A using anti-DYRK1A antibody (A00878-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: rat skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DYRK1A antigen affinity purified polyclonal antibody (Catalog # A00878-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DYRK1A at approximately 100 kDa. The expected band size for DYRK1A is at 86 kDa.

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Figure 2. IF analysis of DYRK1A using anti-DYRK1A antibody (A00878-1).
DYRK1A was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DYRK1A Antibody (A00878-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For DYRK1A (Source: Uniprot.org, NCBI)

Alternative Names

dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A; DYRK1; DYRK1A; MNBEC 2.7.110EC 2.7.12; MNBH DYRK1A DYRK, DYRK1, HP86, MNB, MNBH, MRD7 dual specificity tyrosine phosphorylation regulated kinase 1A dual specificity tyrosine-phosphorylation-regulated kinase 1A|MNB/DYRK protein kinase|dual specificity YAK1-related kinase|dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A|mnb protein kinase homolog hp86|protein kinase minibrain homolog|serine/threonine kinase MNB|serine/threonine-specific protein kinase

For more info on DYRK1A, check out the DYRK1A Infographic

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In this infographic, you will see the following information for DYRK1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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