Click image to see more details
Product Info Summary
| SKU: | A01234-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Dysferlin Antibody
SKU/Catalog Number
A01234-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Dysferlin Antibody (Catalog # A01234-1). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dysferlin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01234-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Dysferlin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01234-1 is reactive to DYSF in Human, Mouse
Applications
A01234-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
237.295kDa
Background of Dysferlin
Key calcium ion sensor involved in the Ca2+-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress By similarity.
Delia J. Hern¨¢ndez-Deviez, Hum. Mol. Genet., Jan 2006; 15: 129 - 142.
Yanchao Huang, FASEB J, Mar 2007; 21: 732 - 742.
Mengfatt Ho, Hum. Mol. Genet., Sep 2004; 13: 1999 - 2010.
Eriko Fujita, Hum. Mol. Genet., Mar 2007; 16: 618 - 629.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from JK cells (Lane 2) and K562 cells (Lane 3), using Dysferlin antiobdy A01234-1. The lane on the left is treated with synthesized peptide.
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Western blot analysis of extracts from K562 cells, using Dysferlin antibody A01234-1.
Protein Target Info & Infographic
Gene/Protein Information For DYSF (Source: Uniprot.org, NCBI)
Gene Name
DYSF
Full Name
Dysferlin
Weight
237.295kDa
Superfamily
ferlin family
Alternative Names
dysferlin; dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive); dystrophy-associated fer-1-like 1; FER1L1Dystrophy-associated fer-1-like protein; Fer-1-like protein 1; FLJ00175; FLJ90168; LGMD2B DYSF FER1L1, LGMD2B, LGMDR2, MMD1 dysferlin dysferlin|dystrophy-associated fer-1-like 1|fer-1-like family member 1|fer-1-like protein 1|limb girdle muscular dystrophy 2B (autosomal recessive)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DYSF, check out the DYSF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DYSF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dysferlin Antibody (A01234-1)
Hello CJ!
No publications found for A01234-1
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Recommended Resources
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Dysferlin Antibody
Question
Is a blocking peptide available for product anti-Dysferlin antibody (A01234-1)?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
We do provide the blocking peptide for product anti-Dysferlin antibody (A01234-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-08
Question
I see that the anti-Dysferlin antibody A01234-1 works with WB, what is the protocol used to produce the result images on the product page?
J. Li
Verified customer
Asked: 2019-04-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-17
Question
I have a question about product A01234-1, anti-Dysferlin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-27
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01234-1 anti-Dysferlin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-27
Question
I was wanting to use your anti-Dysferlin antibody for WB for mouse liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2017-05-22
Answer
As indicated on the product datasheet, A01234-1 anti-Dysferlin antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-22
