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Product Info Summary
| SKU: | M00063-4 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-E Cadherin Rabbit Monoclonal Antibody
SKU/Catalog Number
M00063-4
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-E Cadherin Rabbit Monoclonal Antibody catalog # M00063-4. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-E Cadherin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00063-4)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EA-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human E Cadherin
Reactive Species
M00063-4 is reactive to CDH1 in Human
Observed Molecular Weight
135 kDa
Calculated molecular weight
97.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00063-4 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Positive Control
IHC: human colon cancer tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:6W dilution for 1 hour at room temperature.
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IHC analysis of E Cadherin using anti-E Cadherin antibody (M00063-4).
E Cadherin was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:5000 rabbit anti-E Cadherin Antibody (M00063-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of E Cadherin using anti-E Cadherin antibody (M00063-4).
E Cadherin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:5000 rabbit anti-E Cadherin Antibody (M00063-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Western blot analysis of E-cadherin expression in MCF7 cell lysate.
Specific Publications For Anti-E Cadherin Rabbit Monoclonal Antibody (M00063-4)
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