Anti-Transcription factor E2F4 E2F4 Antibody

Product Name

Anti-Transcription factor E2F4 E2F4 Antibody

View all E2F-4 Antibodies

SKU/Catalog Number

PA2156

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Transcription factor E2F4 E2F4 Antibody catalog # PA2156. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Transcription factor E2F4 E2F4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2156)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human E2F4, different from the related mouse and rat sequences by four amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2156 is reactive to E2F4 in Human, Rat

Applications

PA2156 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

44 kDa

Calculated molecular weight

43.96kDa

Background of E2F-4

Transcription factor E2F4 is a protein that in humans is encoded by the E2F4 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. This gene is mapped to 16q22.1. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

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Anti-E2F4 antibody, PA2156, Western blotting
All lanes: Anti E2F4 (PA2156) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD

Gene/Protein Information For E2F4 (Source: Uniprot.org, NCBI)

Alternative Names

E2F transcription factor 4, p107/p130-binding; E2F4; E2F-4; E2F-4p107/p130-binding protein; transcription factor E2F4 E2F4 E2F-4 E2F transcription factor 4 transcription factor E2F4|E2F transcription factor 4, p107/p130-binding|p107/p130-binding protein

For more info on E2F4, check out the E2F4 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for E2F4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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