Anti-E2F5 Antibody Picoband™

E2F5 antibody

Boster Bio Anti-E2F5 Antibody Picoband™ catalog # A05265-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A05265-1
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, Flow Cytometry, WB

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Product Name

Anti-E2F5 Antibody Picoband™

View all E2F5 Antibodies

SKU/Catalog Number

A05265-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-E2F5 Antibody Picoband™ catalog # A05265-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-E2F5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05265-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human E2F5 recombinant protein (Position: C121-Y346).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05265-1 is reactive to E2F5 in Human

Applications

A05265-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Background of E2F5

Transcription factor E2F5 is a protein that in humans is encoded by the E2F5 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For E2F5 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q15329

Gene ID

1875

Gene Name

E2F5

Full Name

Transcription factor E2F5

Weight

37.61kDa

Superfamily

E2F/DP family

Alternative Names

E2F transcription factor 5, p130-binding; E2F-5; transcription factor E2F5 E2F5 E2F-5 E2F transcription factor 5 transcription factor E2F5|E2F transcription factor 5, p130-binding

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on E2F5, check out the E2F5 Infographic

E2F5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for E2F5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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Conjugation

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Size

Total: $315

Size:100 μg

SKU:A05265-1

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Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.

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A05265-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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