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Product Info Summary
| SKU: | M01713 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01713
BM5136 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal Antibody catalog # M01713. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01713)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABED-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human EAAT2
Reactive Species
M01713 is reactive to SLC1A2 in Human, Mouse, Rat
Observed Molecular Weight
70 kDa
Calculated molecular weight
62.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01713 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of EAAT2 expression in HeLa cell lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Specific Publications For Anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal Antibody (M01713)
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal Antibody
Question
I understand that the actual sequence is confidential. But please let me know the sequence is in intra- or extra-cellular domains?
Verified customer
Asked: 2022-07-19
Answer
Thank you for your questions. The immunogen sequence for M01713 is VAPPPDEEANATSA. It is in extracellular domain. Please feel free to contact us for any questions you have.
Boster Scientific Support
Answered: 2022-07-19
Question
According to your website, this is applicable to IP and WB. In WB, proteins are denatured. I want to make sure that the antibody binds to native protein (not denatured). Is this IP native or denatured proteins? Do you have any data on other applications, such as FCM, IHC, etc.?
Verified customer
Asked: 2022-07-19
Answer
Thank you for your inquiry. RIPA lysis buffer was used to prepare protein samples for IP. The proteins can be basically considered to have natural protein structure or close to native protein structure. Other applications such as FCM, IHC, etc. are not recommended.
Boster Scientific Support
Answered: 2022-07-19
Question
I am interested in to test anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713 on human brain for research purposes, then I may be interested in using anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
The products we sell, including anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-18
Question
I have a customer who would like to reorder M01713. Do you have 5 vials of lot# 0011812m131? What is the expiration date?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
We have 5 vials of M01713 from lot#0011812M13120. Please write down the lot# on your order when you place it to us. We can only send them to you around January 22, 2018 due to spring festival holiday. Contact us freely for any other questions.
Boster Scientific Support
Answered: 2019-10-16
Question
Would anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713 work for IP with brain?
G. Yang
Verified customer
Asked: 2019-01-04
Answer
According to the expression profile of brain, SLC1A2 is highly expressed in brain. So, it is likely that anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713 will work for IP with brain.
Boster Scientific Support
Answered: 2019-01-04
Question
See below the WB image, lot number and protocol we used for brain using anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-16
Question
I see that the anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-04-11
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-04-11
Question
I was wanting to use your anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody for IP for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
M. Bhatt
Verified customer
Asked: 2018-01-08
Answer
You can see on the product datasheet, M01713 anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-08
Question
Is this M01713 anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody reactive to the isotypes of SLC1A2?
Verified Customer
Verified customer
Asked: 2017-08-02
Answer
The immunogen of M01713 anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody is A synthesized peptide derived from human EAAT2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-02
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713. Let me know if you need anything else.
B. Kulkarni
Verified customer
Asked: 2014-04-11
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-04-11
