Anti-EAAT3/SLC1A1 Antibody Picoband™

Product Name

Anti-EAAT3/SLC1A1 Antibody Picoband™

View all EAAT3 Antibodies

SKU/Catalog Number

A02367-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EAAT3/SLC1A1 Antibody Picoband™ catalog # A02367-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EAAT3/SLC1A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02367-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human EAAT3/SLC1A1 recombinant protein (Position: Q160-F524).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A02367-1 is reactive to SLC1A1 in Human, Mouse, Rat

Applications

A02367-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

57.1kDa

Background of EAAT3

Solute carrier family 1 member 1, also called SLC1A1, is a protein that in humans is encoded by the SLC1A1 gene. By Southern analysis of a panel of human/rodent somatic cell hybrids and by fluorescence in situ hybridization (FISH), this gene is mapped to 9p24.2. This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are though to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of EAAT3/SLC1A1 using anti-EAAT3/SLC1A1 antibody (A02367-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAAT3/SLC1A1 antigen affinity purified polyclonal antibody (Catalog # A02367-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EAAT3/SLC1A1 at approximately 70KD. The expected band size for EAAT3/SLC1A1 is at 70KD.

Gene/Protein Information For SLC1A1 (Source: Uniprot.org, NCBI)

Alternative Names

EAAC1excitatory amino acid carrier 1; EAAT3excitatory amino acid transporter 3; Excitatory amino-acid carrier 1; Neuronal and epithelial glutamate transporter; Sodium-dependent glutamate/aspartate transporter 3; solute carrier family 1 (neuronal/epithelial high affinity glutamatetransporter, system Xag), member 1; Solute carrier family 1 member 1 SLC1A1 DCBXA, EAAC1, EAAT3, SCZD18 solute carrier family 1 member 1 excitatory amino acid transporter 3|excitatory amino acid carrier 1|neuronal and epithelial glutamate transporter|sodium-dependent glutamate/aspartate transporter 3|solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1

For more info on SLC1A1, check out the SLC1A1 Infographic

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In this infographic, you will see the following information for SLC1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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