Anti-EB1/MAPRE1 Antibody Picoband™

Product Name

Anti-EB1/MAPRE1 Antibody Picoband™

View all Mapre1 Antibodies

SKU/Catalog Number

A02070-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-EB1/MAPRE1 Antibody Picoband™ catalog # A02070-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-EB1/MAPRE1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02070-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human EB1/MAPRE1 recombinant protein (Position: A145-Y268).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02070-1 is reactive to Mapre1 in Human, Mouse, Rat

Applications

A02070-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

35 kDa

Calculated molecular weight

30.016kDa

Background of Mapre1

Microtubule-associated protein RP/EB family member 1 is a protein that in humans is encoded by the MAPRE1 gene. The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of EB1/MAPRE1 using anti-EB1/MAPRE1 antibody (A02070-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human Ramos whole cell lysates,
Lane 5: human 293T whole cell lysates,
Lane 6: human Caco-2 whole cell lysates,
Lane 7: human HEL whole cell lysates,
Lane 8: human SiHa whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EB1/MAPRE1 antigen affinity purified polyclonal antibody (Catalog # A02070-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EB1/MAPRE1 at approximately 35 kDa. The expected band size for EB1/MAPRE1 is at 30 kDa.

Click image to see more details

Figure 2. Western blot analysis of EB1/MAPRE1 using anti-EB1/MAPRE1 antibody (A02070-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat stomach tissue lysates,
Lane 3: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EB1/MAPRE1 antigen affinity purified polyclonal antibody (Catalog # A02070-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EB1/MAPRE1 at approximately 35 kDa. The expected band size for EB1/MAPRE1 is at 30 kDa.

Click image to see more details

Figure 3. Flow Cytometry analysis of SiHa cells using anti-MAPRE1 antibody (A02070-1).
Overlay histogram showing SiHa cells stained with A02070-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAPRE1 Antibody (A02070-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Mapre1 (Source: Uniprot.org, NCBI)

Alternative Names

adenomatous polyposis coli-binding protein EB1; APC-binding protein EB1; EB1MGC129946; End-binding protein 1; MGC117374; microtubule-associated protein RP/EB family member 1; microtubule-associated protein, RP/EB family, member 1 Mapre1|5530600P05Rik, AI462499, AI504412, AW260097, BIM, BIM1p, D2Ertd459, D2Ertd459e, Eb1|microtubule-associated protein, RP/EB family, member 1|microtubule-associated protein RP/EB family member 1|APC-binding protein EB1|adenomatosis polyposis coli binding protein Eb1|end-binding protein 1

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