Anti-ECH1 Antibody

Product Name

Anti-ECH1 Antibody

View all ECH1 Antibodies

SKU/Catalog Number

A07818

Size

100ul

Form

Liquid

Description

Boster Bio Anti-ECH1 Antibody catalog # A07818. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ECH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07818)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human USP13.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07818 is reactive to ECH1 in Human, Mouse, Rat

Applications

A07818 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

35.816kDa

Background of ECH1

Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.

Timms K.M., Gene 217:101-106(1998).
Mural R.J., Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Dephoure N., Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2000
IHC: 1:50-1:200

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-ECH1 Antibody A07818

Western blot analysis of extracts of various cell lines

Gene/Protein Information For ECH1 (Source: Uniprot.org, NCBI)

Alternative Names

delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; delta3,5-delta2,4-dienoyl-CoA isomerase; dienoyl-CoA isomerase; EC 5.3.3.-; enoyl CoA hydratase 1, peroxisomal; enoyl Coenzyme A hydratase 1, peroxisomal; HPXEL; peroxisomal enoyl-CoA hydratase 1 ECH1 HPXEL enoyl-CoA hydratase 1 delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial|delta3,5-delta2,4-dienoyl-CoA isomerase|delta3,5-delta2,4-dienoyl-coenzyme A isomerase|dienoyl-CoA isomerase|enoyl Coenzyme A hydratase 1, peroxisomal|enoyl-CoA hydratase 1, peroxisomal|epididymis secretory sperm binding protein|peroxisomal enoyl-CoA hydratase 1

For more info on ECH1, check out the ECH1 Infographic

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In this infographic, you will see the following information for ECH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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