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Product Info Summary
| SKU: | A07966-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-ECSIT Antibody (C-term)
SKU/Catalog Number
A07966-1
Size
80 µl
Description
Boster Bio Anti-ECSIT Antibody (C-term) (Catalog # A07966-1). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ECSIT Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07966-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ECSIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-425 amino acids from the C-terminal region of human ECSIT.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07966-1 is reactive to ECSIT in Human
Reconstitution
Calculated molecular weight
49148 Da
Background of Ecsit
Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity). Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07966-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
Validation Images & Assay Conditions
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Fluorescent confocal image of Hela cell stained with ECSIT Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0. 1%, 10 min), then incubated with ECSIT primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ECSIT immunoreactivity is localized to Cytoplasm and Nucleus significantly.
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ECSIT Antibody (C-term) (Cat. #A07966-1) western blot analysis in Jurkat,ZR-75-1 cell line lysates (35ug/lane). This demonstrates the ECSIT antibody detected the ECSIT protein (arrow).
Protein Target Info & Infographic
Gene/Protein Information For ECSIT (Source: Uniprot.org, NCBI)
Gene Name
ECSIT
Full Name
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
Weight
49148 Da
Superfamily
ECSIT family
Alternative Names
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, Protein SITPEC, ECSIT Ecsit|Sit, Sitpec|ECSIT signalling integrator|evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial|ECSIT homolog|signaling intermediate in Toll pathway-evolutionarily conserved
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ECSIT, check out the ECSIT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ECSIT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ECSIT Antibody (C-term) (A07966-1)
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