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Product Info Summary
| SKU: | M16505 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-EEF1A1P5 Antibody (C-Term)
SKU/Catalog Number
M16505
Size
50 µl
Description
Boster Bio Anti-EEF1A1P5 Antibody (C-Term) (Catalog # M16505). Tested in WB, Flow Cytometry, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-EEF1A1P5 Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M16505)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This EEF1A1P5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 430-462 amino acids from human EEF1A1P5.
Reactive Species
M16505 is reactive to EEF1A1P5 in Human
Calculated molecular weight
50.2 kDa
Background of EEF1A1P5
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M16505 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000-1:2000
FC: 1:25
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling EEF1A1P5 with M16505 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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All lanes : Anti-EEF1A1P5 Antibody (C-Term) at 1:1000-1:2000 dilution
Lane 1: U-2OS whole cell lysate
Lane 2: 293 whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: PC-3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing HepG2 cells stained with M16505 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M16505, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed..
Specific Publications For Anti-EEF1A1P5 Antibody (C-Term) (M16505)
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