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Product Info Summary
| SKU: | A11920-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-EFHD1 Antibody
SKU/Catalog Number
A11920-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-EFHD1 Antibody (Catalog # A11920-1). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
EFHD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-EFHD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11920-1)
Host
Rabbit
Contents
EFHD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
EFHD1 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human EFHD1. The immunogen is located within the first 50 amino acids of EFHD1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
GZF1 antibody is human specific. At least four isoforms of GZF1 are known to exist.
Reactive Species
A11920-1 is reactive to EFHD1 in Human, Mouse, Rat
Applications
A11920-1 is guaranteed for ELISA, IF, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
26.928kDa
Background of EFHD1
EFHD1, also known as Swiprosin-2 or SWS2, is an EF-hand and coiled-coil-containing adaptor protein identified in a subtractive hybridization study using a neuronal cell line established from the cerebellum of an adult p53-null mouse, however further study indicated no difference in normal mice. Its mRNA is widely expressed, with its expression in brain undetectable at embryonic stages, with increasing levels from postnatal to adult development. In situ hybridization showed expression in neurons but not white matter of the cerebellum and cerebrum. EHFD1 is also highly expressed in testes, ovary, and the collecting ducts of the kidney, suggesting that in non-neuronal cells, EFHD1 may be involved in gametogenesis and water-reabsorbtion.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
EFHD1 antibody can be used for detection of EFHD1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunoflourescence starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of EFHD1 in human spleen tissue lysate with EFHD1 antibody at (A) 2 and (B) 4 μg/mL.
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Immunofluorescence of EFHD1 in rat spleen tissue with EFHD1 antibody at 5 μg/mL.
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Immunofluorescence of EFHD1 in Rat Spleen cells with EFHD1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For EFHD1 (Source: Uniprot.org, NCBI)
Gene Name
EFHD1
Full Name
EF-hand domain-containing protein D1
Weight
26.928kDa
Alternative Names
DKFZp781H0842; EF-hand domain family, member D1; EF-hand domain-containing protein 1; EF-hand domain-containing protein D1; member D1; MST133; MSTP133; swiprosin-2 EFHD1 MST133, MSTP133, PP3051, SWS2 EF-hand domain family member D1 EF-hand domain-containing protein D1|EF-hand domain-containing protein 1|swiprosin-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EFHD1, check out the EFHD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EFHD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EFHD1 Antibody (A11920-1)
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No publications found for A11920-1
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