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Product Info Summary
| SKU: | M00378 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-EGF Rabbit Monoclonal Antibody
SKU/Catalog Number
M00378
BM5086 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-EGF Rabbit Monoclonal Antibody catalog # M00378. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EGF Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00378)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABOF-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human EGF
Reactive Species
M00378 is reactive to EGF in Human
Observed Molecular Weight
160 kDa
Calculated molecular weight
134.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00378 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
Positive Control
WB: human urine sample
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of EGF expression in human urine sample lysate.
Specific Publications For Anti-EGF Rabbit Monoclonal Antibody (M00378)
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Customer Q&As
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16 Customer Q&As for Anti-EGF Rabbit Monoclonal Antibody
Question
We have observed staining in human renal medulla. Any tips? Is anti-EGF Rabbit Monoclonal antibody supposed to stain renal medulla positively?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
From literature renal medulla does express EGF. From Uniprot.org, EGF is expressed in renal medulla, kidney, teratocarcinoma, colon, among other tissues. Regarding which tissues have EGF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Kidney, Pubmed ID: 3491360
Teratocarcinoma, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-03-31
Question
Does anti-EGF Rabbit Monoclonal antibody M00378 work on horse IP with kidney?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
Our lab technicians have not validated anti-EGF Rabbit Monoclonal antibody M00378 on horse. You can run a BLAST between horse and the immunogen sequence of anti-EGF Rabbit Monoclonal antibody M00378 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse kidney in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-10
Question
I see that the anti-EGF Rabbit Monoclonal antibody M00378 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-15
Question
Is there a BSA free version of anti-EGF Rabbit Monoclonal antibody M00378 available?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-EGF Rabbit Monoclonal antibody M00378 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-03
Question
Is a blocking peptide available for product anti-EGF Rabbit Monoclonal antibody (M00378)?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
We do provide the blocking peptide for product anti-EGF Rabbit Monoclonal antibody (M00378). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-09
Question
I was wanting to use your anti-EGF Rabbit Monoclonal antibody for WB for human renal medulla on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human renal medulla identification?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
As indicated on the product datasheet, M00378 anti-EGF Rabbit Monoclonal antibody has been tested for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human renal medulla in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for renal medulla using anti-EGF Rabbit Monoclonal antibody M00378. Let me know if you need anything else.
R. Wu
Verified customer
Asked: 2019-07-03
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-03
Question
We are currently using anti-EGF Rabbit Monoclonal antibody M00378 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
T. Li
Verified customer
Asked: 2019-06-04
Answer
The anti-EGF Rabbit Monoclonal antibody (M00378) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-04
Question
We need to test anti-EGF Rabbit Monoclonal antibody M00378 on human renal medulla for research purposes, then I may be interested in using anti-EGF Rabbit Monoclonal antibody M00378 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
The products we sell, including anti-EGF Rabbit Monoclonal antibody M00378, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-17
Question
Will anti-EGF Rabbit Monoclonal antibody M00378 work for WB with renal medulla?
Verified Customer
Verified customer
Asked: 2018-10-04
Answer
According to the expression profile of renal medulla, EGF is highly expressed in renal medulla. So, it is likely that anti-EGF Rabbit Monoclonal antibody M00378 will work for WB with renal medulla.
Boster Scientific Support
Answered: 2018-10-04
Question
Can you help my question with product M00378, anti-EGF Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Wu
Verified customer
Asked: 2018-09-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00378 anti-EGF Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-27
Question
Is this M00378 anti-EGF Rabbit Monoclonal antibody reactive to the isotypes of EGF?
Verified Customer
Verified customer
Asked: 2017-08-09
Answer
The immunogen of M00378 anti-EGF Rabbit Monoclonal antibody is A synthesized peptide derived from human EGF. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-09
Question
We have tried in the past anti-EGF Rabbit Monoclonal antibody for IP on teratocarcinoma a few months ago. I am using human, and I plan to use the antibody for WB next. My lab would like examining teratocarcinoma as well as colon in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
L. Wu
Verified customer
Asked: 2016-10-25
Answer
I viewed the website and datasheets of our anti-EGF Rabbit Monoclonal antibody and I see that M00378 has been validated on human in both IP and WB. Thus M00378 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-10-25
Question
See below the WB image, lot number and protocol we used for renal medulla using anti-EGF Rabbit Monoclonal antibody M00378. Please let me know if you require anything else.
R. Krishna
Verified customer
Asked: 2015-10-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-10-08
Question
Would M00378 anti-EGF Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
V. Roberts
Verified customer
Asked: 2015-09-09
Answer
It shows on the product datasheet, M00378 anti-EGF Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-09-09
Question
My boss were content with the WB result of your anti-EGF Rabbit Monoclonal antibody. However we have been able to see positive staining in teratocarcinoma membrane using this antibody. Is that expected? Could you tell me where is EGF supposed to be expressed?
G. Johnson
Verified customer
Asked: 2014-02-06
Answer
From what I have seen in literature, teratocarcinoma does express EGF. Generally EGF expresses in membrane. Regarding which tissues have EGF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Kidney, Pubmed ID: 3491360
Teratocarcinoma, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2014-02-06
