Anti-EGR2 Antibody

EGR2 antibody

Boster Bio Anti-EGR2 Antibody catalog # PA2178. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA2178
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-EGR2 Antibody

View all EGR2 Antibodies

SKU/Catalog Number

PA2178

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EGR2 Antibody catalog # PA2178. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EGR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2178)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human EGR2, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2178 is reactive to EGR2 in Human, Mouse, Rat

Applications

PA2178 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

53 kDa

Calculated molecular weight

50.302kDa

Background of EGR2

Early growth response protein 2, also called EGR2 or E3 SUMO-protein ligase EGR2, is a protein that in humans is encoded by the EGR2 gene. This gene is mapped to 10q21.3. The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 own transcriptional activity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For EGR2 (Source: Uniprot.org, NCBI)

Gene Name

EGR2

Full Name

E3 SUMO-protein ligase EGR2

Weight

50.302kDa

Superfamily

EGR C2H2-type zinc-finger protein family

Alternative Names

AT591; CMT1D; CMT4E; early growth response 2 (Krox-20 homolog, Drosophila); early growth response 2; early growth response protein 2; EGR-2; FLJ14547; Krox-20 homolog, Drosophila; KROX-20, Drosophila, homolog (early growth response-2); KROX20DKFZp686J1957; Zinc finger protein Krox-20 EGR2 AT591, CHN1, CMT1D, CMT4E, KROX20 early growth response 2 E3 SUMO-protein ligase EGR2|E3 SUMO-protein transferase ERG2|KROX-20, Drosophila, homolog (early growth response-2)|early growth response protein 2|zinc finger protein Krox-20

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EGR2, check out the EGR2 Infographic

EGR2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EGR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-EGR2 Antibody

Question

Is this PA2178 anti-EGR2 antibody reactive to the isotypes of EGR2?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

The immunogen of PA2178 anti-EGR2 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human EGR2(153-168aa TMSQTQPDLDHLYSPP), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-05-01

Question

Will anti-EGR2 antibody PA2178 work for WB with brain testis?

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

According to the expression profile of brain testis, EGR2 is highly expressed in brain testis. So, it is likely that anti-EGR2 antibody PA2178 will work for WB with brain testis.

Boster Scientific Support

Answered: 2019-11-18

Question

Do you have a BSA free version of anti-EGR2 antibody PA2178 available?

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-EGR2 antibody PA2178 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-23

Question

I have attached the WB image, lot number and protocol we used for brain testis using anti-EGR2 antibody PA2178. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-23

Question

Would PA2178 anti-EGR2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

It shows on the product datasheet, PA2178 anti-EGR2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-25

Question

We have been able to see staining in rat leukemic t-cell. Are there any suggestions? Is anti-EGR2 antibody supposed to stain leukemic t-cell positively?

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

From what I have seen in literature leukemic t-cell does express EGR2. From what I have seen in Uniprot.org, EGR2 is expressed in tibial nerve, brain testis, endometrium, leukemic t-cell, among other tissues. Regarding which tissues have EGR2 expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 14702039
Endometrium, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332

Boster Scientific Support

Answered: 2019-07-03

Question

Our lab were satisfied with the WB result of your anti-EGR2 antibody. However we have observed positive staining in brain testis nucleus. using this antibody. Is that expected? Could you tell me where is EGR2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

According to literature, brain testis does express EGR2. Generally EGR2 expresses in nucleus. Regarding which tissues have EGR2 expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 14702039
Endometrium, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332

Boster Scientific Support

Answered: 2019-06-07

Question

Does anti-EGR2 antibody PA2178 work on primate WB with endometrium?

Verified Customer

Verified customer

Asked: 2019-05-17

Answer

Our lab technicians have not tested anti-EGR2 antibody PA2178 on primate. You can run a BLAST between primate and the immunogen sequence of anti-EGR2 antibody PA2178 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate endometrium in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-17

Question

I see that the anti-EGR2 antibody PA2178 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-04-24

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-04-24

Question

My question regarding product PA2178, anti-EGR2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-10-15

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2178 anti-EGR2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-10-15

Question

I am interested in to test anti-EGR2 antibody PA2178 on rat brain testis for research purposes, then I may be interested in using anti-EGR2 antibody PA2178 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

C. Johnson

Verified customer

Asked: 2018-07-31

Answer

The products we sell, including anti-EGR2 antibody PA2178, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-07-31

Question

Is a blocking peptide available for product anti-EGR2 antibody (PA2178)?

Verified Customer

Verified customer

Asked: 2018-05-03

Answer

We do provide the blocking peptide for product anti-EGR2 antibody (PA2178). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-05-03

Question

I was wanting to use your anti-EGR2 antibody for WB for rat brain testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain testis identification?

Verified Customer

Verified customer

Asked: 2017-07-31

Answer

As indicated on the product datasheet, PA2178 anti-EGR2 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-07-31

Question

My question regards using your anti-EGR2 antibody for myelination studies. Has this antibody been tested with western blotting on nih3t3 cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-06-12

Answer

We appreciate your inquiry. This PA2178 anti-EGR2 antibody is tested on nih3t3 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-06-12

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain testis using anti-EGR2 antibody PA2178. Let me know if you need anything else.

E. Miller

Verified customer

Asked: 2015-05-22

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-05-22

Question

We are currently using anti-EGR2 antibody PA2178 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

G. Johnson

Verified customer

Asked: 2013-08-02

Answer

The anti-EGR2 antibody (PA2178) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-08-02

Order DetailsPrice
PA2178

100μg

$370
PA2178-10ug

10μg sample (liquid)

$99
PA2178-Biotin

100 μg Biotin conjugated

$570
PA2178-Cy3

100 μg Cy3 conjugated

$570
PA2178-Dylight488

100 μg Dylight488 conjugated

$570
PA2178-Dylight550

100 μg Dylight550 conjugated

$570
PA2178-Dylight594

100 μg Dylight594 conjugated

$570
PA2178-FITC

100 μg FITC conjugated

$570
PA2178-HRP

100 μg HRP conjugated

$570
PA2178-APC

100 μg APC conjugated

$670
PA2178-PE

100 μg PE conjugated

$670
PA2178-carrier-free

Carrier Free

$370

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