Product Info Summary
SKU: | A05757-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-EHHADH Antibody
SKU/Catalog Number
A05757-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-EHHADH Antibody catalog # A05757-2. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EHHADH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05757-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human EHHADH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05757-2 is reactive to EHHADH in Human, Mouse, Rat
Applications
A05757-2 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
79.495kDa
Background of EHHADH
The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene.
Hoefler G., Genomics 19:60-67(1994).
Cherkaoui-Malki M., Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
Totoki Y., Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-EHHADH Antibody A05757-2
Western blot (WB) analysis of EHHADH polyclonal antibody at 1:500 dilutionLane1:Jurkat cell lysate
Lane2:Raw264.7 cell lysate
Lane3:H9C2 cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For EHHADH (Source: Uniprot.org, NCBI)
Gene Name
EHHADH
Full Name
Peroxisomal bifunctional enzyme
Weight
79.495kDa
Alternative Names
enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase; enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase; LBFP; LBP; L-PBE; PBFEMGC120586; peroxisomal bifunctional enzyme3,2-trans-enoyl-CoA isomerase; peroxisomal enoyl-CoA hydratase; peroxisomal EHHADH ECHD, FRTS3, L-PBE, LBFP, LBP, MFE1, PBFE enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase peroxisomal bifunctional enzyme|3,2-trans-enoyl-CoA isomerase|L-3-hydroxyacyl-CoA dehydrogenase|L-bifunctional protein, peroxisomal|PBE|enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase|enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase|multifunctional enzyme 1|peroxisomal enoyl-CoA hydratase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EHHADH, check out the EHHADH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EHHADH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EHHADH Antibody (A05757-2)
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