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EIF2A antibody (A02418-1) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of Caco-2 cells using anti-EIF2A antibody (A02418-1).
Overlay histogram showing Caco-2 cells stained with A02418-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF2A Antibody (A02418-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Caco-2 cells stained with A02418-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF2A Antibody (A02418-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-EIF2A Antibody Picoband®
EIF2A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
EIF2A antibody was positively detected by WB in:
human Caco-2 whole cell, human K562 whole cell, human U251 whole cell, rat PC-12 tissue, human Hela- WT whole cell lysates, human Hela-EIF2A KO whole cell lysates
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
EIF2A antibody was positively detected by IHC in:
human pancreas tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
EIF2A antibody was positively detected by FC in:
Caco-2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-EIF2A Antibody Picoband®
View all EIF2A Antibodies
View all EIF2A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human EIF2A recombinant protein (Position: H24-I585). Human EIF2A shares 92.5% amino acid (aa) sequence identity with mouse EIF2A.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
65 kDa
Calculated Molecular Weight
65.0 kDa
Background of EIF2A
This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Cite This Product
Anti-EIF2A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02418-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
96%
High Homology
Rabbit
95%
High Homology
Sheep
95%
High Homology
Cow
94%
High Homology
Mouse
93%
High Homology
Rat
92%
High Homology
Zebrafish
70%
Moderate Homology
Drosophila
40%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for EIF2A antibody
Immunohistochemistry (IHC) protocol for EIF2A antibody
Flow Cytometry (FC) protocol for EIF2A antibody
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Product Promise
We promise Anti-EIF2A Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A02418-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026