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- Table of Contents
Designing a Western blot for EIF2A? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the EIF2A Western Blot GuideFacts about Eukaryotic translation initiation factor 2A.
In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40S subunits in a codon- dependent fashion, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner. .
| Human | |
|---|---|
| Gene Name: | EIF2A |
| Uniprot: | Q9BY44 |
| Entrez: | 83939 |

| Belongs to: |
|---|
| WD repeat EIF2A family |

EIF-2; EIF-2A; EIF2AEIF2; EIF-2alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; subunit 1 (alpha, 35kD )
Mass (kDA):
64.99 kDA

| Human | |
|---|---|
| Location: | 3q25.1 |
| Sequence: | 3; NC_000003.12 (150546678..150586016) |
Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.




PMID: 12133843 by Zoll W.L., et al. Characterization of mammalian eIF2A and identification of the yeast homolog.
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