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Product Info Summary
| SKU: | A08134 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-eIF2B gamma EIF2B3 Antibody
SKU/Catalog Number
A08134
Size
100μl
Form
Liquid
Description
Boster Bio Anti-eIF2B gamma EIF2B3 Antibody catalog # A08134. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF2B gamma EIF2B3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08134)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from eIF2Bγ . at AA range: 240-320
Reactive Species
A08134 is reactive to EIF2B3 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
50.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08134 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using eIF2Bγ Polyclonal Antibody diluted at 1:1000
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Western Blot analysis of K562 cells using eIF2Bγ Polyclonal Antibody diluted at 1:1000
Specific Publications For Anti-eIF2B gamma EIF2B3 Antibody (A08134)
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Customer Q&As
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4 Customer Q&As for Anti-eIF2B gamma EIF2B3 Antibody
Question
I see that the anti-eIF2B gamma antibody A08134 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-22
Question
We are currently using anti-eIF2B gamma antibody A08134 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-13
Answer
The anti-eIF2B gamma antibody (A08134) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-13
Question
Would anti-eIF2B gamma antibody A08134 work for WB with liver?
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
According to the expression profile of liver, EIF2B3 is highly expressed in liver. So, it is likely that anti-eIF2B gamma antibody A08134 will work for WB with liver.
Boster Scientific Support
Answered: 2018-07-30
Question
Does anti-eIF2B gamma antibody A08134 work on canine WB with erythroleukemia?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
Our lab technicians have not validated anti-eIF2B gamma antibody A08134 on canine. You can run a BLAST between canine and the immunogen sequence of anti-eIF2B gamma antibody A08134 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-26
