Product Info Summary
SKU: | A04191 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-EIF2C3/Argonaute 3 AGO3 Antibody
View all Argonaute 3 Antibodies
SKU/Catalog Number
A04191
Size
100ul
Form
Liquid
Description
Boster Bio Anti-EIF2C3/Argonaute 3 AGO3 Antibody catalog # A04191. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EIF2C3/Argonaute 3 AGO3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04191)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human 60S Ribosomal Protein L10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04191 is reactive to AGO3 in Human, Mouse, Rat
Applications
A04191 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
97.36kDa
Background of Argonaute 3
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L10E family of ribosomal proteins. It is located in the cytoplasm. In vitro studies have shown that the chicken protein can bind to c-Jun and can repress c-Jun-mediated transcriptional activation, but these activities have not been demonstrated in vivo. This gene was initially identified as a candidate for a Wilms tumor suppressor gene, but later studies determined that this gene is not involved in the suppression of Wilms tumor. This gene has been referred to as 'laminin receptor homolog' because a chimeric transcript consisting of sequence from this gene and sequence from the laminin receptor gene was isolated; however, it is not believed that this gene encodes a laminin receptor. Alternative splicing results in multiple transcript variants. This gene also uses multiple polyA signals, with the 3'-most polyA signal overlapping the deoxyribonuclease I-like 1 gene on the opposite strand. This gene is co-transcribed with the small nucleolar RNA gene U70, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Dowdy S.F., Nucleic Acids Res. 19:5763-5769(1991).
van den Ouweland A.M.W., Hum. Mol. Genet. 1:269-273(1992).
Kaneko K., Hum. Mol. Genet. 1:529-533(1992).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Immunocytochemistry staining of AGO3 using Anti-EIF2C3/Argonaute 3 AGO3 Antibody (A04191).
ICC staining EIF2C3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 2. Immunocytochemistry staining of AGO3 using Anti-EIF2C3/Argonaute 3 AGO3 Antibody (A04191).
ICC staining EIF2C3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeProtein Target Info & Infographic
Gene/Protein Information For AGO3 (Source: Uniprot.org, NCBI)
Gene Name
AGO3
Full Name
Protein argonaute-3
Weight
97.36kDa
Superfamily
argonaute family
Alternative Names
AGO3argonaute 3; argonaute3; eIF2C 3; eIF-2C 3; Eukaryotic translation initiation factor 2C 3; eukaryotic translation initiation factor 2C, 3; FLJ12765; hAgo3; MGC86946; protein argonaute-3 AGO3 EIF2C3 argonaute RISC catalytic component 3 protein argonaute-3|argonaute 3, RISC catalytic component|eukaryotic translation initiation factor 2C, 3|hAgo3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AGO3, check out the AGO3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AGO3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EIF2C3/Argonaute 3 AGO3 Antibody (A04191)
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No publications found for A04191
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