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Product Info Summary
| SKU: | A05989-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-SOCS5 Antibody Picoband®
SKU/Catalog Number
A05989-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SOCS5 Antibody Picoband® catalog # A05989-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SOCS5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05989-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SOCS5 recombinant protein (Position: M386-K536).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05989-1 is reactive to SOCS5 in Human, Mouse, Rat
Observed Molecular Weight
61 kDa
Calculated molecular weight
61.2 kDa
Background of SOCS5
Suppressor of cytokine signaling 5 is a protein that in humans is encoded by the SOCS5 gene. It is mapped to 2p21. The protein encoded by this gene contains a SH2 domain and a SOCS BOX domain. The protein thus belongs to the suppressor of cytokine signaling (SOCS) family, also known as STAT-induced STAT inhibitor (SSI) protein family. SOCS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The specific function of this protein has not yet been determined. Two alternatively spliced transcript variants encoding an identical protein have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05989-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human U87 whole cell, human U2OS whole cell, human A549 whole cell, human K562 whole cell, human 293T whole cell, human Hela whole cell, rat brain tissue, mouse brain tissue
IHC: human ovarican cancer tissue, human prostatic acinar adenocarcinoma tissue
Validation Images & Assay Conditions
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Western blot analysis of SOCS5 using anti-SOCS5 antibody (A05989-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human 293T whole cell lysates,
Lane 6: human Hela whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS5 antigen affinity purified polyclonal antibody (A05989-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SOCS5 at approximately 61 kDa. The expected band size for SOCS5 is at 61 kDa.
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IHC analysis of SOCS5 using anti-SOCS5 antibody (A05989-1).
SOCS5 was detected in a paraffin-embedded section of human ovarican cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOCS5 Antibody (A05989-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of SOCS5 using anti-SOCS5 antibody (A05989-1).
SOCS5 was detected in a paraffin-embedded section of human prostatic acinar adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOCS5 Antibody (A05989-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-SOCS5 Antibody Picoband® (A05989-1)
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1 Customer Q&As for Anti-SOCS5 Antibody Picoband®
Question
We are currently using anti-SOCS5 antibody A05989-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
The anti-SOCS5 antibody (A05989-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-04
