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Product Info Summary
| SKU: | A02238 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-eIF3L/Eif3S6Ip Antibody
SKU/Catalog Number
A02238
Size
100μl
Form
Liquid
Description
Boster Bio Anti-eIF3L/Eif3S6Ip Antibody catalog # A02238. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF3L/Eif3S6Ip Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02238)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human IF3EI. AA range:1-50
Reactive Species
A02238 is reactive to EIF3L in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
66.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02238 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using eIF3L Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from HUVEC cells, using IF3EI Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-eIF3L/Eif3S6Ip Antibody (A02238)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-eIF3L/Eif3S6Ip Antibody
Question
My question regarding product A02238, anti-eIF3L/Eif3S6Ip antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-22
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02238 anti-eIF3L/Eif3S6Ip antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-22
Question
Is there a BSA free version of anti-eIF3L/Eif3S6Ip antibody A02238 available?
Verified Customer
Verified customer
Asked: 2017-07-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-eIF3L/Eif3S6Ip antibody A02238 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-24
Question
Is a blocking peptide available for product anti-eIF3L/Eif3S6Ip antibody (A02238)?
Verified Customer
Verified customer
Asked: 2017-05-17
Answer
We do provide the blocking peptide for product anti-eIF3L/Eif3S6Ip antibody (A02238). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-05-17
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix colon using anti-eIF3L/Eif3S6Ip antibody A02238. Let me know if you need anything else.
D. Carter
Verified customer
Asked: 2016-08-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-08-12
Question
Is this A02238 anti-eIF3L/Eif3S6Ip antibody reactive to the isotypes of EIF3L?
K. Parker
Verified customer
Asked: 2015-01-27
Answer
The immunogen of A02238 anti-eIF3L/Eif3S6Ip antibody is Synthesized peptide derived from the N-terminal region of human eIF3L. at AA rangle: 10-90. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-01-27
Question
Here is the WB image, lot number and protocol we used for cervix colon using anti-eIF3L/Eif3S6Ip antibody A02238. Please let me know if you require anything else.
R. Jackson
Verified customer
Asked: 2014-05-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-05-06
Question
We are currently using anti-eIF3L/Eif3S6Ip antibody A02238 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on horse tissues as well?
K. Zhang
Verified customer
Asked: 2013-06-20
Answer
The anti-eIF3L/Eif3S6Ip antibody (A02238) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-20
