Product Info Summary
SKU: | M00968 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00968
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody catalog # M00968. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00968)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IGG-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human eIF4EBP1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00968 is reactive to EIF4EBP1 in Human, Mouse, Rat
Applications
M00968 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
150 kDa
Calculated molecular weight
12.58kDa
Background of 4EBP1
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of eIF4EBP1 expression in K562 cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using eIF4EBP1 Antibody.
Click image to see more details
Immunofluorescent analysis of HeLa cells, using eIF4EBP1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For EIF4EBP1 (Source: Uniprot.org, NCBI)
Gene Name
EIF4EBP1
Full Name
Eukaryotic translation initiation factor 4E-binding protein 1
Weight
12.58kDa
Superfamily
eIF4E-binding protein family
Alternative Names
4EBP1; 4E-BP1; eIF4E-binding protein 1; EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1; eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHAS-IMGC4316; Phosphorylated heat- and acid-stable protein regulated by insulin 1 EIF4EBP1 4E-BP1, 4EBP1, BP-1, PHAS-I eukaryotic translation initiation factor 4E binding protein 1 eukaryotic translation initiation factor 4E-binding protein 1|eIF4E-binding protein 1|phosphorylated heat- and acid-stable protein regulated by insulin 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF4EBP1, check out the EIF4EBP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF4EBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody (M00968)
Hello CJ!
M00968 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu R,Zhang Y,Li A,Ma Y,Cai W,Song L,Xie Y,Zhou S,Cao W,Tang X.LY‑294002 enhances the chemosensitivity of liver cancer to oxaliplatin by blocking the PI3K/AKT/HIF‑1α pathway.Mol Med Rep.2021 Jul;24(1):508.doi:10.3892/mmr.2021.12147.Epub 2021 May 13.PMID:33982772.
Species: Human
M00968 usage in article: APP:WB, SAMPLE:HEPG-2 CELL, DILUTION:NA
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody
Question
We are currently using anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody M00968 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-25
Answer
The anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody (M00968) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-25
Question
Would anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody M00968 work for Flow Cytometry with body of pancreas?
Verified Customer
Verified customer
Asked: 2018-12-24
Answer
According to the expression profile of body of pancreas, EIF4EBP1 is highly expressed in body of pancreas. So, it is likely that anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody M00968 will work for Flow Cytometry with body of pancreas.
Boster Scientific Support
Answered: 2018-12-24
Question
Can you help my question with product M00968, anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Evans
Verified customer
Asked: 2017-09-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00968 anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-20