Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody

4EBP1 antibody

Boster Bio Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody catalog # M00968. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M00968
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody

View all 4EBP1 Antibodies

SKU/Catalog Number

M00968

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody catalog # M00968. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00968)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

IGG-5

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human eIF4EBP1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00968 is reactive to EIF4EBP1 in Human, Mouse, Rat

Applications

M00968 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

150 kDa

Calculated molecular weight

12.58kDa

Background of 4EBP1

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For EIF4EBP1 (Source: Uniprot.org, NCBI)

Gene Name

EIF4EBP1

Full Name

Eukaryotic translation initiation factor 4E-binding protein 1

Weight

12.58kDa

Superfamily

eIF4E-binding protein family

Alternative Names

4EBP1; 4E-BP1; eIF4E-binding protein 1; EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1; eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHAS-IMGC4316; Phosphorylated heat- and acid-stable protein regulated by insulin 1 EIF4EBP1 4E-BP1, 4EBP1, BP-1, PHAS-I eukaryotic translation initiation factor 4E binding protein 1 eukaryotic translation initiation factor 4E-binding protein 1|eIF4E-binding protein 1|phosphorylated heat- and acid-stable protein regulated by insulin 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EIF4EBP1, check out the EIF4EBP1 Infographic

EIF4EBP1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EIF4EBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00968 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Xu R,Zhang Y,Li A,Ma Y,Cai W,Song L,Xie Y,Zhou S,Cao W,Tang X.LY‑294002 enhances the chemosensitivity of liver cancer to oxaliplatin by blocking the PI3K/AKT/HIF‑1α pathway.Mol Med Rep.2021 Jul;24(1):508.doi:10.3892/mmr.2021.12147.Epub 2021 May 13.PMID:33982772.
Species: Human
M00968 usage in article: APP:WB, SAMPLE:HEPG-2 CELL, DILUTION:NA

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3 Customer Q&As for Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody

Question

We are currently using anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody M00968 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-04-25

Answer

The anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody (M00968) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-04-25

Question

Would anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody M00968 work for Flow Cytometry with body of pancreas?

Verified Customer

Verified customer

Asked: 2018-12-24

Answer

According to the expression profile of body of pancreas, EIF4EBP1 is highly expressed in body of pancreas. So, it is likely that anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody M00968 will work for Flow Cytometry with body of pancreas.

Boster Scientific Support

Answered: 2018-12-24

Question

Can you help my question with product M00968, anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

A. Evans

Verified customer

Asked: 2017-09-20

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00968 anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-09-20

Order DetailsPrice
M00968

100μL

$370
M00968-30ul

30μL sample

$99
M00968-carrier-free

Carrier Free

$370

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M00968
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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