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Product Info Summary
| SKU: | M00968 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00968
BM4851 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody catalog # M00968. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00968)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IGG-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human eIF4EBP1
Reactive Species
M00968 is reactive to EIF4EBP1 in Human, Mouse, Rat
Observed Molecular Weight
18 kDa
Calculated molecular weight
12.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00968 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: K562 cell
IHC: human breast carcinoma tissue
ICC/IF: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of eIF4EBP1 expression in K562 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using eIF4EBP1 Antibody.
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Immunofluorescent analysis of HeLa cells, using eIF4EBP1 Antibody.
Specific Publications For Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody (M00968)
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3 Customer Q&As for Anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal Antibody
Question
We are currently using anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody M00968 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-25
Answer
The anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody (M00968) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-25
Question
Would anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody M00968 work for Flow Cytometry with body of pancreas?
Verified Customer
Verified customer
Asked: 2018-12-24
Answer
According to the expression profile of body of pancreas, EIF4EBP1 is highly expressed in body of pancreas. So, it is likely that anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody M00968 will work for Flow Cytometry with body of pancreas.
Boster Scientific Support
Answered: 2018-12-24
Question
Can you help my question with product M00968, anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Evans
Verified customer
Asked: 2017-09-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00968 anti-eIF4EBP1/4Ebp1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-20
