Product Info Summary
SKU: | A02450 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-EKLF (W318) KLF1 Antibody
SKU/Catalog Number
A02450
Size
100ul
Form
Liquid
Description
Boster Bio Anti-EKLF (W318) KLF1 Antibody catalog # A02450. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EKLF (W318) KLF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02450)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human BMP-1 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02450 is reactive to KLF1 in Human, Mouse, Rat
Applications
A02450 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
38.221kDa
Background of KLF1
Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A.
Ladias J.A.A., Science 251:561-565(1991).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Wang L.H., Gene Expr. 1:207-216(1991).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-EKLF (W318) KLF1 Antibody A02450
Western blot (WB) analysis of EKLF (W318) pAb at 1:500 dilutionLane1:C6 whole cell lysate(40ug)
Lane2:SP2/0 whole cell lysate(40ug)
Lane3:MCF-7 whole cell lysate(40ug)
Lane4:K562 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For KLF1 (Source: Uniprot.org, NCBI)
Gene Name
KLF1
Full Name
Krueppel-like factor 1
Weight
38.221kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
EKLFerythroid-specific transcription factor EKLF; Erythroid krueppel-like transcription factor; erythroid Kruppel-like factor; HBFQTL6; INLU; Krueppel-like factor 1; Kruppel-like factor 1 (erythroid); monoclonal A3D8 KLF1 EKLF, EKLF/KLF1 Kruppel like factor 1 Krueppel-like factor 1|erythroid Kruppel-like factor|erythroid krueppel-like transcription factor|erythroid-specific transcription factor EKLF|truncated Kruppel-like factor 1|truncated erythroid Kruppel-like factor EKLF/KLF1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLF1, check out the KLF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EKLF (W318) KLF1 Antibody (A02450)
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No publications found for A02450
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