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Product Info Summary
| SKU: | A01027-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IP, WB |
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Product info
Product Name
Anti-ELF3 Antibody Picoband®
SKU/Catalog Number
A01027-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ELF3 Antibody Picoband® catalog # A01027-2. Tested in WB, IP, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ELF3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01027-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human ELF3 recombinant protein (Position: Q48-L314). Human ELF3 shares 87.3% and 90% amino acid (aa) sequence identity with mouse and rat ELF3, respectively.
Reactive Species
A01027-2 is reactive to ELF3 in Human
Observed Molecular Weight
50 kDa
Calculated molecular weight
41.5 kDa
Background of ELF3
Enables DNA-binding transcription activator activity, RNA polymerase II-specific and sequence-specific double-stranded DNA binding activity. Involved in inflammatory response; negative regulation of DNA-templated transcription; and positive regulation of transcription by RNA polymerase II. Located in Golgi apparatus; cytosol; and nucleoplasm.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01027-2 is guaranteed for ELISA, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
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Western blot analysis of ELF3 using anti-ELF3 antibody (A01027-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELF3 antigen affinity purified polyclonal antibody (A01027-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ELF3 at approximately 50 kDa. The expected band size for ELF3 is at 41 kDa.
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Immunoprecipitating ELF3 in A549 whole cell lysate.
Western blot analysis of ELF3 using anti-ELF3 antibody (A01027-2).
Lane 1: A549 whole cell lysates (30ug),
Lane 2: Rabbit control IgG instead of anti-ELF3 antibody in A549 whole cell lysate,
Lane 3: anti-ELF3 antibody (2μg) + A549 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ELF3 antigen affinity purified polyclonal antibody (A01027-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ELF3 at approximately 50 kDa. The expected band size for ELF3 is at 41 kDa.
Specific Publications For Anti-ELF3 Antibody Picoband® (A01027-2)
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