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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-KAT9/ELP3 Antibody Picoband™ (monoclonal, 8B4)
Boster Bio Anti-KAT9/ELP3 Antibody Picoband™ (monoclonal, 8B4) catalog # M02833-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KAT9/ELP3 Antibody Picoband™ (monoclonal, 8B4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02833-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
A synthetic peptide corresponding to a sequence of human KAT9/ELP3 (RDPTKFQHQGFGMLLMEEAERIAREEH).
M02833-1 is reactive to ELP3 in Human, Mouse, Rat
M02833-1 is guaranteed for WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of KAT9/ELP3 using anti-KAT9/ELP3 antibody (M02833-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human U20S whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-KAT9/ELP3 antigen affinity purified monoclonal antibody (Catalog # M02833-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for KAT9/ELP3 at approximately 62KD. The expected band size for KAT9/ELP3 is at 62KD.
Gene/Protein Information For ELP3 (Source: Uniprot.Org, NCBI)
Elongator complex protein 3
EC 184.108.40.206; elongation protein 3 homolog (S. cerevisiae); elongator complex protein 3; hELP3; KAT9FLJ10422 ELP3 KAT9 elongator acetyltransferase complex subunit 3 elongator complex protein 3|elongation protein 3 homolog|protein lysine acetyltransferase ELP3|tRNA uridine(34) acetyltransferase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ELP3, check out the ELP3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ELP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for M02833-1
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