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Product Info Summary
| SKU: | M02833-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4)
SKU/Catalog Number
M02833-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4) catalog # M02833-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02833-1)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Monoclonal
Clone Number
8B4
Isotype
Mouse IgG2a
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human KAT9/ELP3, identical to the related mouse sequence.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M02833-1 is reactive to ELP3 in Human, Mouse, Rat
Observed Molecular Weight
62 kDa
Calculated molecular weight
62.3 kDa
Background of ELP3
Elongator complex protein 3, also named KAT9, is aproteinthat in humans is encoded by theELP3gene. It is mapped to 8p21.1. ELP3 is the catalytichistone acetyltransferasesubunit of theRNA polymeraseII elongator complex, which is a component of the RNA polymerase II (Pol II)holoenzymeand is involved in transcriptional elongation. ELP3 supports the migration and branching ofprojection neuronsthroughacetylationof alpha-tubulin in the developingcerebral cortex. In mammals, ELP3 is important for paternalDNA demethylationafterfertilization.ELP3 is potentially involved in cellular redoxhomeostasisby mediating the acetylation ofglucose-6-phosphate dehydrogenase. Besides, ELP3 may play a role inchromatin remodelingand is involved in acetylation ofhistones H3and probablyH4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02833-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Positive Control
WB: human K562 whole cell, human U20S whole cell, human HepG2 whole cell, human U87 whole cell, rat lung tissue, rat PC-12 whole cell, mouse lung tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of KAT9/ELP3 using anti-KAT9/ELP3 antibody (M02833-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human U20S whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-KAT9/ELP3 antigen affinity purified monoclonal antibody (Catalog # M02833-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for KAT9/ELP3 at approximately 62KD. The expected band size for KAT9/ELP3 is at 62KD.
Specific Publications For Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4) (M02833-1)
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