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Product Info Summary
| SKU: | A13581 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-EMR4 ADGRE4P Antibody
SKU/Catalog Number
A13581
Size
100μl
Form
Liquid
Description
Boster Bio Anti-EMR4 ADGRE4P Antibody catalog # A13581. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EMR4 ADGRE4P Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13581)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human EMR4P. AA range:101-150
Reactive Species
A13581 is reactive to ADGRE4P in Human, Monkey
Calculated molecular weight
50.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13581 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using EMR4 Polyclonal Antibody
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Western Blot analysis of COLO205 cells using EMR4 Polyclonal Antibody
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Western blot analysis of lysates from HUVEC cells, COS7 cells, HeLa cells, and COLO205 cells, using EMR4 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-EMR4 ADGRE4P Antibody (A13581)
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Customer Q&As
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6 Customer Q&As for Anti-EMR4 ADGRE4P Antibody
Question
Does A13581 anti-EMR4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
V. Bhatt
Verified customer
Asked: 2020-03-23
Answer
It shows on the product datasheet, A13581 anti-EMR4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-23
Question
Is a blocking peptide available for product anti-EMR4 antibody (A13581)?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
We do provide the blocking peptide for product anti-EMR4 antibody (A13581). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-08-08
Question
Is this A13581 anti-EMR4 antibody reactive to the isotypes of ADGRE4P?
L. Collins
Verified customer
Asked: 2017-11-09
Answer
The immunogen of A13581 anti-EMR4 antibody is Synthesized peptide derived from the Internal region of human EMR4. at AA rangle: 80-160. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-09
Question
Do you have a BSA free version of anti-EMR4 antibody A13581 available?
Verified Customer
Verified customer
Asked: 2017-10-16
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-EMR4 antibody A13581 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-16
Question
I have a question about product A13581, anti-EMR4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Miller
Verified customer
Asked: 2015-02-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13581 anti-EMR4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-02-18
Question
I see that the anti-EMR4 antibody A13581 works with WB, what is the protocol used to produce the result images on the product page?
C. Carter
Verified customer
Asked: 2014-03-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-03-20
