Anti-ENaC alpha Monoclonal Antibody

Product Name

Anti-ENaC alpha Monoclonal Antibody

View all epithelial Sodium Channel alpha Antibodies

SKU/Catalog Number

M01413

Size

100ug

Form

liquid

Description

Boster Bio Anti-ENaC alpha Monoclonal Antibody catalog # M01413. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ENaC alpha Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01413)

Host

Mouse

Contents

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

2G4

Isotype

IgG2b

Immunogen

Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~85kDa.

Reactive Species

M01413 is reactive to SCNN1A in Mouse, Rat

Applications

M01413 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

75.704kDa

Background of epithelial Sodium Channel alpha

The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:1000); IHC (1:150); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

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Figure 1. IHC analysis of Scnn1a using anti-Scnn1a antibody (M01413).
Scnn1a was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Scnn1a Antibody (M01413) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

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Figure 2. IHC analysis of Scnn1a using anti-Scnn1a antibody (M01413).
Scnn1a was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Scnn1a Antibody (M01413) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

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Figure 3. Western blot analysis of Scnn1a using anti-Scnn1a antibody (M01413).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Scnn1a antigen affinity purified polyclonal antibody (Catalog # M01413) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Scnn1a.

Gene/Protein Information For SCNN1A (Source: Uniprot.org, NCBI)

Alternative Names

alpha ENaC-2; alpha-ENaC; alpha-NaCH; amiloride-sensitive epithelial sodium channel alpha subunit; amiloride-sensitive sodium channel subunit alpha 2; amiloride-sensitive sodium channel subunit alpha; BESC2; ENaCa; ENaCalpha; Epithelial Na(+) channel subunit alpha; FLJ21883; nasal epithelial sodium channel alpha subunit; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA; SCNN1; sodium channel, nonvoltage-gated 1 alpha SCNN1A BESC2, ENaCa, ENaCalpha, LIDLS3, SCNEA, SCNN1 sodium channel epithelial 1 subunit alpha amiloride-sensitive sodium channel subunit alpha|alpha ENaC-2|alpha-ENaC|alpha-NaCH|amiloride-sensitive epithelial sodium channel alpha subunit|amiloride-sensitive sodium channel subunit alpha 2|epithelial Na(+) channel subunit alpha|nasal epithelial sodium channel alpha subunit|nonvoltage-gated sodium channel 1 subunit alpha|sodium channel epithelial 1 alpha subunit|sodium channel, non voltage gated 1 alpha subunit|sodium channel, non-voltage-gated 1 alpha|sodium channel, nonvoltage-gated 1 alpha

For more info on SCNN1A, check out the SCNN1A Infographic

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In this infographic, you will see the following information for SCNN1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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