Anti-EndoPDI Monoclonal Antibody

Product Name

Anti-EndoPDI Monoclonal Antibody

View all TXNDC5 Antibodies

SKU/Catalog Number

M05772

Size

100ug

Form

liquid

Description

Boster Bio Anti-EndoPDI Monoclonal Antibody catalog # M05772. Tested in ELISA, IP, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EndoPDI Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05772)

Host

Mouse

Contents

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

2E7/7

Isotype

IgG2a

Immunogen

Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the Human EndoPDI protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~48kDa.

Reactive Species

M05772 is reactive to TXNDC5 in Human

Applications

M05772 is guaranteed for ELISA, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

47.629kDa

Background of TXNDC5

Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia (1).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:200); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EndoPDI Monoclonal Antibody, Clone 2E7/7 (M05772) . Tissue: Cervical Cancer cell line (HeLa) . Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EndoPDI Monoclonal Antibody (M05772) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.

Gene/Protein Information For TXNDC5 (Source: Uniprot.org, NCBI)

Alternative Names

EndoPDI; ERp46; FLJ90810; HCC-2; member 15; MGC3178; PDIA15; thioredoxin domain containing 5 (endoplasmic reticulum); thioredoxin domain containing 5; thioredoxin domain-containing protein 5; thioredoxin related protein; TLP46; TXNDC5 TXNDC5 ENDOPDI, ERP46, HCC-2, HCC2, PDIA15, STRF8, UNQ364 thioredoxin domain containing 5 thioredoxin domain-containing protein 5|ER protein 46|endoplasmic reticulum protein ERp46|endoplasmic reticulum resident protein 46|endothelial protein disulphide isomerase|epididymis secretory sperm binding protein|protein disulfide isomerase family A, member 15|thioredoxin domain containing 5 (endoplasmic reticulum)|thioredoxin related protein|thioredoxin-like protein p46

For more info on TXNDC5, check out the TXNDC5 Infographic

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In this infographic, you will see the following information for TXNDC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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