Product Info Summary
| SKU: | A03110-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-ENPP2 Antibody (Center)
SKU/Catalog Number
A03110-1
Size
50 µl
Description
Boster Bio Anti-ENPP2 Antibody (Center) (Catalog # A03110-1). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ENPP2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03110-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ENPP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 amino acids from the Central region of human ENPP2.
Reactive Species
A03110-1 is reactive to ENPP2 in Human
Calculated molecular weight
99.0 kDa
Background of ENPP2
ENPP2 functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This protein stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The protein is secreted and further processed to make the biologically active form.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03110-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of lysates from human placenta and plasma tissue lysate (from left to right), using ENPP2 Antibody (Center). A03110-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Western blot analysis of (arrow) using rabbit polyclonal ENPP2 Antibody (Center) (Cat. #A03110-1). 293T cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the gene.
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Anti-ENPP2 Antibody (Center) at 1:2000 dilution + Human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 99 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Anti-ENPP2 Antibody (Center) at 1:2000 dilution + Human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 99 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
ENPP2 Antibody (Center) (Cat. #A03110-1) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-ENPP2 Antibody (Center) (A03110-1)
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