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Product Info Summary
| SKU: | A03110-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-ENPP2 Antibody (Center)
View all ENPP-2/Autotaxin Antibodies
SKU/Catalog Number
A03110-1
Size
50 µl
Description
Boster Bio Anti-ENPP2 Antibody (Center) (Catalog # A03110-1). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ENPP2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03110-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ENPP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 amino acids from the Central region of human ENPP2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03110-1 is reactive to ENPP2 in Human
Reconstitution
Calculated molecular weight
98994 Da
Background of ENPP-2/Autotaxin
ENPP2 functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This protein stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The protein is secreted and further processed to make the biologically active form.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03110-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of lysates from human placenta and plasma tissue lysate (from left to right), using ENPP2 Antibody (Center). A03110-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Western blot analysis of (arrow) using rabbit polyclonal ENPP2 Antibody (Center) (Cat. #A03110-1). 293T cell lysates (２ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the gene.
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Anti-ENPP2 Antibody (Center) at 1:2000 dilution + Human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 99 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-ENPP2 Antibody (Center) at 1:2000 dilution + Human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 99 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
ENPP2 Antibody (Center) (Cat. #A03110-1) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For ENPP2 (Source: Uniprot.org, NCBI)
Gene Name
ENPP2
Full Name
Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
Weight
98994 Da
Superfamily
nucleotide pyrophosphatase/phosphodiesterase family
Alternative Names
Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, E-NPP 2, Autotaxin, Extracellular lysophospholipase D, LysoPLD, ENPP2, ATX, PDNP2 ENPP2 ATX, ATX-X, AUTOTAXIN, LysoPLD, NPP2, PD-IALPHA, PDNP2 ectonucleotide pyrophosphatase/phosphodiesterase 2 ectonucleotide pyrophosphatase/phosphodiesterase family member 2|E-NPP 2|autotaxin-t|extracellular lysophospholipase D|phosphodiesterase I/nucleotide pyrophosphatase 2|plasma lysophospholipase D
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ENPP2, check out the ENPP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ENPP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ENPP2 Antibody (Center) (A03110-1)
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