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Product Info Summary
| SKU: | A07914-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-ENTPD2 Antibody (N-term)
SKU/Catalog Number
A07914-1
Size
80 µl
Description
Boster Bio Anti-ENTPD2 Antibody (N-term) (Catalog # A07914-1). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ENTPD2 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07914-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ENTPD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the N-terminal region of human ENTPD2.
Reactive Species
A07914-1 is reactive to ENTPD2 in Human
Calculated molecular weight
53.7 kDa
Background of ENTPD2
ENTPD2 is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07914-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-ENTPD2 Antibody at 1:1000 dilution
Lane 1: human heart lysate
Lane 2: 293 whole cell lysate
Lane 3: U-87 MG whole cell lysate
Lane 4: human brain lysate
Lane 5: human skeletal muscle lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 54 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-ENTPD2 Antibody (N-term) at 1:2000 dilution
Lane 1: H.heart lysate
Lane 2: 293 whole cell lysate
Lane 3: U-87 MG whole cell lysate
Lane 4: H.brain whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 54 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of ENTPD2 Antibody (N-term) in K562 cell line lysates (35ug/lane). ENTPD2 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with ENTPD2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Overlay histogram showing K562 cells stained with A07914-1 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A07914-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Specific Publications For Anti-ENTPD2 Antibody (N-term) (A07914-1)
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