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Product Info Summary
| SKU: | A04794 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-EPAC2 RAPGEF4 Antibody
SKU/Catalog Number
A04794
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-EPAC2 RAPGEF4 Antibody (Catalog # A04794). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
EPAC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-EPAC2 RAPGEF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04794)
Host
Rabbit
Contents
EPAC2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
EPAC2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human EPAC2. The immunogen is located within the first 50 amino acids of EPAC2.
Cross-reactivity
At least two isoforms of EPAC2 are known to exist; this antibody will detect only the larger isoform. EPAC2 antibody is predicted to not cross-react with EPAC2
Reactive Species
A04794 is reactive to RAPGEF4 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
115.5 kDa
Background of RAPGEF4
EPAC2, also known as Rap guanine nuclear exchange factor 4 and cAMPGEF-II, is belongs to a family of cyclic adenosine monophosphate (cAMP) binding proteins with guanine nucleotide exchange factor. Like the related protein EPAC1, EPAC2 signaling plays a role in numerous cellular processes such as integrin-mediated cell adhesion, muscle contraction, learning and memory, cell proliferation, and inflammation. Recent evidence suggests that EPAC2 induces synapse remodeling and depression, with mutations in its gene being found in patients with autism.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04794 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
EPAC2 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of EPAC2 in rat liver tissue lysate with EPAC2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of EPAC3 in mouse liver tissue with EPAC3 antibody at 2.5 μg/mL.
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Immunofluorescence of EPAC2 in mouse liver tissue with EPAC2 antibody at 20 μg/mL.
Specific Publications For Anti-EPAC2 RAPGEF4 Antibody (A04794)
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