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- Table of Contents
EPCAM antibody (M30950-1) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Immunofluorescent analysis of Hela cells, using EpCAM/Trop1 Antibody at 1:50 dilution.
Designing a Western blot for EPCAM? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the EPCAM Western Blot GuideBundle Save
Anti-EpCAM/Trop1 Rabbit Monoclonal Antibody
EPCAM antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
EPCAM antibody was positively detected by WB in:
human Caco-2 whole cell, human MCF-7 whole cell, rat small intestine tissue, mouse small intestine tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
EPCAM antibody was positively detected by IHC in:
mouse small intestine tissue, rat small intestine tissue, human breast tissue, human colon tissue, human colon cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB
1:500-2000
IHC
1:50-200
ICC/IF
1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-EpCAM/Trop1 Rabbit Monoclonal Antibody
View all EPCAM Antibodies
View all EPCAM Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CIB-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human EpCAM
Observed Molecular Weight
35-40 kDa
Calculated Molecular Weight
34.9 kDa
Cite This Product
Anti-EpCAM/Trop1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30950-1)
Protocols
Western Blot protocol for EPCAM antibody
Immunohistochemistry (IHC) protocol for EPCAM antibody
Publications
Application:
Species:
A self-amplifying cuproptosis nanomedicine to overcome immunosuppression by blocking tumor-derived exosomes for enhancing lung cancer immunotherapy
Published: 2026-01-02
Journal: JOURNAL OF CONTROLLED RELEASE
Application: WB
Impact Factor: 11.50
Species: Mouse
PMID: 41485488
Review
Have you used Anti-EpCAM/Trop1 Rabbit Monoclonal Antibody?
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1 Review For Anti-EpCAM/Trop1 Rabbit Monoclonal Antibody
Anti-CD326/EPCAM Antibody (M30950-1) specifically detected EPCAM (~34 kDa) in HepG2 cells by Western blot, producing clean and stable target bands without obvious non-specific binding, which validates its high specificity and reliable performance.
| SKU | M30950-1 |
|---|---|
| Application | Western Blot |
| Sample | Human HepG2 cell lysates |
| Sample Processing Description | Following lentiviral transduction, the HepG2 cell line was harvested by centrifugation. The cell pellet was lysed using enhanced RIPA buffer, denatured in 5× SDS loading buffer at 98°C for 8 minutes, and then loaded onto an SDS-PAGE gel. |
| Other Reagents | Enhanced RIPA Lysis Buffer (Catalog No. AR0102, Boster Biological Technology) |
| Primary Antibody | EpCAM/Trop1 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated anti-rabbit IgG secondary antibody (Catalog No. BA1054, Boster Biological Technology) |
| Secondary Incubation | 1:2000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:MiniChemi 500 |
| Results Summary | In this experiment, the HepG2 cell line was used as the sample. The resulting EPCAM protein band appeared at approximately 34 kDa; it was clear, showed stable expression, and exhibited no significant non-specific binding. These results further confirm that the Anti-CD326/EPCAM antibody possesses excellent recognition capability and high specificity, consistent with the expected experimental outcomes. |
Rong Fang, Capital Medical University
Verified customer
Submitted 2026-11-05
Product Promise
We promise Anti-EpCAM/Trop1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M30950-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 21, 2026