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Product Info Summary
| SKU: | M01494-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-EPHA5 Antibody
SKU/Catalog Number
M01494-1
Size
80 µl
Description
Boster Bio Anti-EPHA5 Antibody (Catalog # M01494-1). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-EPHA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01494-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
46CT61.6.4
Isotype
IgG1κ
Immunogen
Purified His-tagged EPHA5 protein (Fragment) was used to produced this monoclonal antibody.
Reactive Species
M01494-1 is reactive to EPHA5 in Human
Calculated molecular weight
114.8 kDa
Background of EPHA5
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01494-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of anti-EPHA5 Monoclonal Antibody by EPHA5 recombinant protein (Fragment). EPHA5 (Fragment) protein (arrow) was detected using the purified Mab. (1:2000)
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Western blot analysis of lysate from U-251 MG cell line, using EphA5 Antibody. M01494-1 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35μg.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with EPHA5 Monoclonal Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Specific Publications For Anti-EPHA5 Antibody (M01494-1)
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