Anti-Ephrin-B2 (Ab-330) EFNB2 Antibody

Product Name

Anti-Ephrin-B2 (Ab-330) EFNB2 Antibody

View all Ephrin-B2 Antibodies

SKU/Catalog Number

A02368-2

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Ephrin-B2 (Ab-330) EFNB2 Antibody (Catalog # A02368-2). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ephrin-B2 (Ab-330) EFNB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02368-2)

Host

Rabbit

Contents

pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Clonality

Polyclonal

Clone Number

1G11

Isotype

IgG

Immunogen

Peptide sequence around aa.328~332 (N-I-Y-Y-K) derived from Human Ephrin B (ephrin-B2).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02368-2 is reactive to EFNB2 in Human, Mouse, Rat

Applications

A02368-2 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

36.923kDa

Background of Ephrin-B2

Ephrin-B2 encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a lycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.

Chrencik JE,et al.( 2006) J Biol Chem;281(38):28185-28192.
Kertesz N, et al. (2006) Blood; 107(6):2330-2338.
Noren NK, et al. (2004) Proc Natl Acad Sci USA; 101(15):5583-5588.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 37kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Immunohistochemistry:1:100~1:200

Validation Images & Assay Conditions

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Immunofluorescent analysis of formalin-fixed, paraffin-embedded hela cells with A02368-2 at 1/75 dilution.A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary.

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Immunofluorescent analysis of formalin-fixed, paraffin-embedded C6 cells with A02368-2 at 1/75 dilution.A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary.

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All lanes : Ephrin-B2(Ab-330) Antibody(A02368-2) at 1/1000 dilutionLane 1 : Mouse spleen tissue

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Figure 2. IHC analysis of EFNB2 using anti-EFNB2 antibody (A02368-2).
EFNB2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EFNB2 Antibody (A02368-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of EFNB2 using anti-EFNB2 antibody (A02368-2).
EFNB2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EFNB2 Antibody (A02368-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 4. IHC analysis of EFNB2 using anti-EFNB2 antibody (A02368-2).
EFNB2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EFNB2 Antibody (A02368-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Click image to see more details

Figure 5. IHC analysis of EFNB2 using anti-EFNB2 antibody (A02368-2).
EFNB2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EFNB2 Antibody (A02368-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For EFNB2 (Source: Uniprot.org, NCBI)

Alternative Names

EFNB2; ELF-2; ephrin B2; EphrinB2; Ephrin-B2; EPLG5; HTKL; Htk-L; LERK5; LERK-5; NLERK-1 EFNB2 EPLG5, HTKL, Htk-L, LERK5 ephrin B2 ephrin-B2|HTK ligand|eph-related receptor tyrosine kinase ligand 5|ligand of eph-related kinase 5

For more info on EFNB2, check out the EFNB2 Infographic

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In this infographic, you will see the following information for EFNB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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