Product Info Summary
SKU: | M01494 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Ephrin type-A receptor 5 EphA5 Monoclonal Antibody
SKU/Catalog Number
M01494
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Ephrin type-A receptor 5 EphA5 Monoclonal Antibody catalog # M01494. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ephrin type-A receptor 5 EphA5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01494)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
8B10B1 / 8B10F5
Isotype
IgG
Immunogen
Purified recombinant fragment of EphA5 (aa620-774) expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M01494 is reactive to EPHA5 in Human
Applications
M01494 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
114.803kDa
Background of EPHA5
TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Ephrin type-A receptor 5 EphA5 Monoclonal Antibody M01494
Western Blot (WB) analysis using EphA5 Monoclonal Antibody against truncated EPHA5-His recombinant protein (1) and truncated EPHA5(aa620-774)-hIgGFc transfected CHO-K1 cell lysate(2).Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For EPHA5 (Source: Uniprot.org, NCBI)
Gene Name
EPHA5
Full Name
Ephrin type-A receptor 5
Weight
114.803kDa
Superfamily
protein kinase superfamily
Alternative Names
Bsk; Cek7; EC 2.7.10; EC 2.7.10.1; Ehk1; EHK-1; EHK1Hek7; EK7; EPH homology kinase 1; Eph homology kinase-1; EPH receptor A5; EphA5; EPH-like kinase 7; ephrin type-A receptor 5; Hek7; receptor protein-tyrosine kinase HEK7; Rek7; TYRO4HEK7CEK7; tyrosine-protein kinase receptor EHK-1 EPHA5 CEK7, EHK-1, EHK1, EK7, HEK7, TYRO4 EPH receptor A5 ephrin type-A receptor 5|EPH homology kinase 1|EPH-like kinase 7|brain-specific kinase|epididymis secretory sperm binding protein|receptor protein-tyrosine kinase HEK7|tyrosine-protein kinase receptor EHK-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPHA5, check out the EPHA5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPHA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ephrin type-A receptor 5 EphA5 Monoclonal Antibody (M01494)
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No publications found for M01494
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