ESR1 · Western blot design guide

ESR1 Western Blot Planning Guide

Plan an ESR1 Western blot around the catalog-observed 66.2 kDa band, image-backed PB9192 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for ESR1 (ESR1): expected band 66.2 kDa, antibody PB9192, and PMC-cited SDS-PAGE protocol steps

Band Protocols Controls Antibodies

ESR1 Western Blot Experimental Design Guide

Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	66.2 kDa
	Observed band	Not reported — verify product WB image
	Gel	12-15%
	Positive control ⓘ	Cervix
	Negative control ⓘ	Appendix

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	66.2 kDa
ⓘ	Localization	Nucleus / Cytoplasm
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Human

Sample controls for ESR1 Western blot

🧪Use Cervix as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.

Positive control: Cervix (High)

Negative control: Appendix (Not detected)

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for ESR1

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Cervix	Reported tissue cells	High	Protein (HPA)	HPA →
Vagina	Reported tissue cells	High	Protein (HPA)	HPA →
Endometrium	Reported tissue cells	High	Protein (HPA)	HPA →
Breast	Reported tissue cells	Medium	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source
Appendix	Reported tissue cells	Not detected	Protein (HPA)	HPA →
Bone marrow	Reported tissue cells	Not detected	Protein (HPA)	HPA →

Section 1

What Is the Expected ESR1 Western Blot Band Size?

Use the product-observed 66.2 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
66.2 kDa	Matches the authoritative product WB observation.
66.2 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected ESR1 appearancePlan around 66.2 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	66.2 kDa; use this as the primary experimental expectation.
Calculated mass	66.2 kDa from UniProt P03372; retain as context.
Gel selection	12-15%; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with PB9192.

Why is my band missing or off?
Situation	Likely cause	Next action
66.2 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt P03372 Human Protein Atlas product datasheet (PB9192)

Experiment evidence

Western blot images showing ESR1 band

PB9192 · ESR1 — observed 66.2 kDa WB validation

PB9192 · ESR1Observed 66.2 kDa · product-specific WB image

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Section 2

Real Curated ESR1 Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Cervix
Gel %	12-15%
Load	20-30 µg total protein per lane
Transfer	Semi-dry, standard transfer
Membrane	0.45 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	PB9192 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 4

Advanced ESR1 Western Blot Tips

Deeper troubleshooting and optimisation questions for ESR1, answered from its protein features.

Which band should guide the blot?

Use 66.2 kDa, the observation attached to the authoritative PB9192 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 66.2 kDa expectation.

Which positive control should I start with?

Start with Cervix, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use Appendix as an orthogonal HPA Not detected candidate.

Which gel should I use?

Use 12-15% consistently across the quick facts, protocol table, and poster.

What transfer method to use for ESR1 Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should PB9192 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC11755959, PMC8725601, PMC8946988.

Boster reagents

ESR1 Western Blot Reagents

Human-reactive ESR1 Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for ESR1 using PB9192; observed band 66.2 kDa

Anti-Estrogen Receptor/ESR1 Antibody Picoband®

Cat # PB9192

Reactivity: Human

View product →

Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.

Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.