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Product Info Summary
|Reactive Species:||Human, Mouse|
|Application:||IF, IHC, ICC, WB|
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Anti-ERCC1 Rabbit Monoclonal Antibody
Boster Bio Anti-ERCC1 Rabbit Monoclonal Antibody catalog # M00388. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERCC1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00388)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human ERCC1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M00388 is reactive to ERCC1 in Human, Mouse
M00388 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
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Western blot analysis of ERCC1 expression in HeLa cell lysate (M00388).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERCC1 monoclonal antibody (Catalog # M00388) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERCC1
Gene/Protein Information For ERCC1 (Source: Uniprot.Org, NCBI)
DNA excision repair protein ERCC-1
COFS4; DNA excision repair protein ERCC-1; ERCC1; excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence); RAD10; UV20 ERCC1 COFS4, RAD10, UV20 ERCC excision repair 1, endonuclease non-catalytic subunit DNA excision repair protein ERCC-1|excision repair cross-complementation group 1|excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ERCC1, check out the ERCC1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ERCC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for M00388
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