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Product Info Summary
| SKU: | A01993-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ERCC4 (A822) Antibody
SKU/Catalog Number
A01993-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ERCC4 (A822) Antibody catalog # A01993-1. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERCC4 (A822) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01993-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Gab 1 around the phosphorylation site of Y659.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01993-1 is reactive to ERCC4 in Human, Mouse, Rat
Applications
A01993-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
104.486kDa
Background of XPF
The protein encoded by Gab1 is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.
Anders Kallin, et al. (2004) J. Biol. Chem ; 279: 17897 - 17904.
Hideto Kameda, et al. (2001) Cell Growth Differ ; 12: 307.
Masaki Osawa, et al. (2002) J. Cell Biol ; 158: 773.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-ERCC4 (A822) Antibody A01993-1
Western blot (WB) analysis of ERCC4 (A822) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate(40ug)
Lane2:MCF-7 whole cell lysate(40ug)
Lane3:U-87MG whole cell lysate(40ug)
Lane4:C6 whole cell lysate(40ug)
Lane5:CT26 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ERCC4 (Source: Uniprot.org, NCBI)
Gene Name
ERCC4
Full Name
DNA repair endonuclease XPF
Weight
104.486kDa
Superfamily
XPF family
Alternative Names
DNA excision repair protein ERCC-4; DNA repair protein complementing XP-F cells; EC 3.1; ERCC11; ERCC4; excision repair cross-complementing rodent repair deficiency, complementationgroup 4; xeroderma pigmentosum, complementation group F; XPF; XPFcomplementing defective, in Chinese hamster ERCC4 ERCC11, FANCQ, RAD1, XFEPS, XPF ERCC excision repair 4, endonuclease catalytic subunit DNA repair endonuclease XPF|DNA excision repair protein ERCC-4|DNA repair protein complementing XP-F cells|excision repair cross-complementation group 4|excision repair cross-complementing rodent repair deficiency, complementation group 4|excision-repair, complementing defective, in Chinese hamster|xeroderma pigmentosum group F-complementing protein|xeroderma pigmentosum, complementation group F
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERCC4, check out the ERCC4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERCC4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ERCC4 (A822) Antibody (A01993-1)
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No publications found for A01993-1
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