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Product Info Summary
| SKU: | A09260 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ERGI3 ERGIC3 Antibody
SKU/Catalog Number
A09260
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ERGI3 ERGIC3 Antibody (Catalog# A09260). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERGI3 ERGIC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09260)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ERGI3. Immunogen sequence location: 321-370
Reactive Species
A09260 is reactive to ERGIC3 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
43.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09260 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Immunofluorescence analysis of HepG2 cells, using ERGI3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from LOVO and NIH/3T3 cells, using ERGI3 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-ERGI3 ERGIC3 Antibody (A09260)
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5 Customer Q&As for Anti-ERGI3 ERGIC3 Antibody
Question
See attached the WB image, lot number and protocol we used for cervix carcinoma using anti-ERGI3 antibody A09260. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-19
Question
We are currently using anti-ERGI3 antibody A09260 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
The anti-ERGI3 antibody (A09260) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-29
Question
Will A09260 anti-ERGI3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
It shows on the product datasheet, A09260 anti-ERGI3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-24
Question
Would anti-ERGI3 antibody A09260 work on bovine WB with brain skin?
Verified Customer
Verified customer
Asked: 2018-02-22
Answer
Our lab technicians have not validated anti-ERGI3 antibody A09260 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-ERGI3 antibody A09260 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain skin in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-22
Question
Is there a BSA free version of anti-ERGI3 antibody A09260 available?
A. Patel
Verified customer
Asked: 2016-04-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ERGI3 antibody A09260 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-04-15
