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Product Info Summary
| SKU: | A07042-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Erlin-2/ERLIN2 Antibody Picoband®
SKU/Catalog Number
A07042-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Erlin-2/ERLIN2 Antibody Picoband® catalog # A07042-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Erlin-2/ERLIN2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07042-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Erlin-2/ERLIN2 recombinant protein (Position: D87-N339).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07042-2 is reactive to ERLIN2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
37.84kDa
Background of SPFH2
Erlin-2 is a protein that in humans is encoded by the ERLIN2 gene. This gene encodes a member of the SPFH domain-containing family of lipid raft-associated proteins. The encoded protein is localized to lipid rafts of the endoplasmic reticulum and plays a critical role in inositol 1,4,5-trisphosphate (IP3) signaling by mediating ER-associated degradation of activated IP3 receptors. Mutations in this gene are a cause of spastic paraplegia-18 (SPG18). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07042-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell, human Hela whole cell, human T-47D whole cell, human U-87MG whole cell, rat kidney tissue, rat testis tissue, rat liver tissue, mouse kidney tissue, mouse testis tissue
ICC/IF: T-47D cell
FCM: Caco-2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Erlin-2/ERLIN2 using anti-Erlin-2/ERLIN2 antibody (A07042-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human T-47D whole cell lysates,
Lane 4: human U-87MG whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 8: rat liver tissue lysates,
Lane 9: mouse kidney tissue lysates,
Lane 10: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Erlin-2/ERLIN2 antigen affinity purified polyclonal antibody (Catalog # A07042-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Erlin-2/ERLIN2 at approximately 43 kDa. The expected band size for Erlin-2/ERLIN2 is at 43 kDa.
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Figure 2. IF analysis of Erlin-2/ERLIN2 using anti-Erlin-2/ERLIN2 antibody (A07042-2).
Erlin-2/ERLIN2 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Erlin-2/ERLIN2 Antibody (A07042-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of Caco-2 cells using anti-Erlin-2/ERLIN2 antibody (A07042-2).
Overlay histogram showing Caco-2 cells stained with A07042-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Erlin-2/ERLIN2 Antibody (A07042-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ERLIN2 (Source: Uniprot.org, NCBI)
Gene Name
ERLIN2
Full Name
Erlin-2
Weight
37.84kDa
Superfamily
band 7/mec-2 family
Alternative Names
Forkhead box protein F1; Forkhead-related activator 1; FREAC-1; Forkhead-related protein FKHL5; Forkhead-related transcription factor 1; FOXF1; FKHL5; FREAC1 ERLIN2 C8orf2, Erlin-2, NET32, SPFH2, SPG18 ER lipid raft associated 2 erlin-2|SPFH domain family, member 2|endoplasmic reticulum lipid raft-associated protein 2|epididymis secretory sperm binding protein|spastic paraplegia 18 (autosomal dominant)|stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERLIN2, check out the ERLIN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERLIN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Erlin-2/ERLIN2 Antibody Picoband® (A07042-2)
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