Anti-Erlin-2/ERLIN2 Antibody Picoband™

Product Name

Anti-Erlin-2/ERLIN2 Antibody Picoband™

View all SPFH2 Antibodies

SKU/Catalog Number

A07042-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Erlin-2/ERLIN2 Antibody Picoband™ catalog # A07042-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Erlin-2/ERLIN2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07042-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Erlin-2/ERLIN2 recombinant protein (Position: D87-N339).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07042-2 is reactive to ERLIN2 in Human, Mouse, Rat

Applications

A07042-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

37.84kDa

Background of SPFH2

Erlin-2 is a protein that in humans is encoded by the ERLIN2 gene. This gene encodes a member of the SPFH domain-containing family of lipid raft-associated proteins. The encoded protein is localized to lipid rafts of the endoplasmic reticulum and plays a critical role in inositol 1,4,5-trisphosphate (IP3) signaling by mediating ER-associated degradation of activated IP3 receptors. Mutations in this gene are a cause of spastic paraplegia-18 (SPG18). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Erlin-2/ERLIN2 using anti-Erlin-2/ERLIN2 antibody (A07042-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human T-47D whole cell lysates,
Lane 4: human U-87MG whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 8: rat liver tissue lysates,
Lane 9: mouse kidney tissue lysates,
Lane 10: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Erlin-2/ERLIN2 antigen affinity purified polyclonal antibody (Catalog # A07042-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Erlin-2/ERLIN2 at approximately 43 kDa. The expected band size for Erlin-2/ERLIN2 is at 43 kDa.

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Figure 2. IF analysis of Erlin-2/ERLIN2 using anti-Erlin-2/ERLIN2 antibody (A07042-2).
Erlin-2/ERLIN2 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Erlin-2/ERLIN2 Antibody (A07042-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of Caco-2 cells using anti-Erlin-2/ERLIN2 antibody (A07042-2).
Overlay histogram showing Caco-2 cells stained with A07042-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Erlin-2/ERLIN2 Antibody (A07042-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For ERLIN2 (Source: Uniprot.org, NCBI)

Alternative Names

C8orf2SPFH domain-containing protein 2; Endoplasmic reticulum lipid raft-associated protein 2; ER lipid raft associated 2; Erlin-2; MGC87072; NET32; SPFH domain family, member 2; SPFH2chromosome 8 open reading frame 2; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2 ERLIN2 C8orf2, Erlin-2, NET32, SPFH2, SPG18 ER lipid raft associated 2 erlin-2|SPFH domain family, member 2|endoplasmic reticulum lipid raft-associated protein 2|epididymis secretory sperm binding protein|spastic paraplegia 18 (autosomal dominant)|stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2

For more info on ERLIN2, check out the ERLIN2 Infographic

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In this infographic, you will see the following information for ERLIN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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