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Product Info Summary
| SKU: | A08034-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-ERLIN1 Antibody Picoband®
SKU/Catalog Number
A08034-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ERLIN1 Antibody Picoband® catalog # A08034-3. Tested in WB, IP applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ERLIN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08034-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ERLIN1.
Reactive Species
A08034-3 is reactive to ERLIN1 in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
39.2 kDa
Background of ERLIN1
The protein encoded by this gene is part of a protein complex that mediates degradation of inositol 1,4,5-trisphosphate receptors in the endoplasmic reticulum. The encoded protein also binds cholesterol and regulates the SREBP signaling pathway, which promotes cellular cholesterol homeostasis. Defects in this gene have been associated with spastic paraplegia 62.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08034-3 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Validation Images & Assay Conditions
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Western blot analysis of ERLIN1 using anti-ERLIN1 antibody (A08034-3).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Hacat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERLIN1 antigen affinity purified polyclonal antibody (A08034-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ERLIN1 at approximately 39 kDa. The expected band size for ERLIN1 is at 39 kDa.
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Immunoprecipitating ERLIN1 in A549 whole cell lysate.
Western blot analysis of ERLIN1 using anti-ERLIN1 antibody (A08034-3).
Lane 1: A549 whole cell lysates (30ug),
Lane 2: Rabbit control IgG instead of anti-ERLIN1 antibody in A549 whole cell lysate,
Lane 3: anti-ERLIN1 antibody (2μg) + A549 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ERLIN1 antigen affinity purified polyclonal antibody (A08034-3) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ERLIN1 at approximately 39 kDa. The expected band size for ERLIN1 is at 39 kDa.
Specific Publications For Anti-ERLIN1 Antibody Picoband® (A08034-3)
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