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Product Info Summary
| SKU: | A05659-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC |
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Product info
Product Name
Anti-ERN2 Antibody
SKU/Catalog Number
A05659-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ERN2 Antibody (Catalog# A05659-1). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05659-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ERN2. Immunogen sequence location: 841-890
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05659-1 is reactive to ERN2 in Human, Mouse
Applications
A05659-1 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
102.48kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using ERN2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of A549 cells, using ERN2 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For ERN2 (Source: Uniprot.org, NCBI)
Gene Name
ERN2
Full Name
Serine/threonine-protein kinase/endoribonuclease IRE2
Weight
102.48kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; endoplasmic reticulum to nucleus signaling 2; endoplasmic reticulum to nucleus signalling 2; Endoplasmic reticulum-to-nucleus signaling 2; ER to nucleus signalling 2; hIRE2p; inositol-requiring 1 beta; Inositol-requiring protein 2; IRE1, S. cerevisiae, homolog of; Ire1-beta; IRE1bIRE1 beta; IRE2; serine/threonine-protein kinase/endoribonuclease IRE2 ERN2 IRE1-BETA, IRE1b, IRE2p, hIRE2p endoplasmic reticulum to nucleus signaling 2 serine/threonine-protein kinase/endoribonuclease IRE2|ER to nucleus signalling 2|IRE1 beta|IRE1, S. cerevisiae, homolog of|endoplasmic reticulum to nucleus signalling 2|inositol-requiring 1 beta|inositol-requiring protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERN2, check out the ERN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ERN2 Antibody (A05659-1)
Hello CJ!
No publications found for A05659-1
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-ERN2 Antibody
Question
Can A05659-1 work for Western Bot?
Verified customer
Asked: 2021-02-21
Answer
The Anti-ERN2 Antibody (A05659-1) is not recommended for WB.
Boster Scientific Support
Answered: 2021-02-22
Question
I have a question about product A05659-1, anti-ERN2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05659-1 anti-ERN2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-29
Question
We are currently using anti-ERN2 antibody A05659-1 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
The anti-ERN2 antibody (A05659-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-06
Question
See attached the WB image, lot number and protocol we used for nasal cavity epithelium using anti-ERN2 antibody A05659-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-18
Question
Will anti-ERN2 antibody A05659-1 work on primate IF with embl:bab21297.1}?
Verified Customer
Verified customer
Asked: 2018-01-18
Answer
Our lab technicians have not tested anti-ERN2 antibody A05659-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-ERN2 antibody A05659-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate embl:bab21297.1} in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-18
Question
Is a blocking peptide available for product anti-ERN2 antibody (A05659-1)?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
We do provide the blocking peptide for product anti-ERN2 antibody (A05659-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-05-31
Question
I was wanting to use your anti-ERN2 antibody for IF for mouse nasal cavity epithelium on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse nasal cavity epithelium identification?
M. Roberts
Verified customer
Asked: 2014-11-04
Answer
As indicated on the product datasheet, A05659-1 anti-ERN2 antibody has been tested for IF, IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse nasal cavity epithelium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-11-04
