Anti-Exonuclease 1 Exo1 Antibody

Product Name

Anti-Exonuclease 1 Exo1 Antibody

View all Exonuclease 1 Antibodies

SKU/Catalog Number

A00536

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Exonuclease 1 Exo1 Antibody catalog # A00536. Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Exonuclease 1 Exo1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00536)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

8H7

Isotype

IgG

Immunogen

Synthesized peptide derived from the N-terminal region of human Exo1. at AA range: 30-110

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00536 is reactive to EXO1 in Human, Mouse

Applications

A00536 is guaranteed for ELISA, IF, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

94.103kDa

Background of Exonuclease 1

ROC1 also known as RING-box protein 1, Rbx1, Regulator of cullins 1, RING finger protein 75, and ZYP protein, is a component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC1 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. The RING-type zinc finger domain is essential for ubiquitin ligase activity. ROC1 probably also stimulates CDC34 autoubiquitination and promotes the neddylation of CUL1 and probably CUL2. ROC1 has a cytoplasmic and nuclear localization and is widely expressed in most tissues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of various cells using Exo1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Click image to see more details

Immunofluorescence analysis of A549 cells, using EXO1 Antibody. The picture on the right is blocked with the synthesized peptide.

Gene/Protein Information For EXO1 (Source: Uniprot.org, NCBI)

Alternative Names

EXOI; exonuclease 1; Exonuclease I; HEX1rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1; hExo1; hExoIEC 3.1 EXO1 HEX1, hExoI exonuclease 1 exonuclease 1|rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1

For more info on EXO1, check out the EXO1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EXO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the EXO1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis