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Product Info Summary
| SKU: | A02065-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-EXT2 Antibody (Center)
SKU/Catalog Number
A02065-1
Size
80 µl
Description
Boster Bio Anti-EXT2 Antibody (Center) (Catalog # A02065-1). Tested in WB, Flow Cytometry, IF, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-EXT2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02065-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This EXT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-209 amino acids from the Central region of human EXT2.
Reactive Species
A02065-1 is reactive to EXT2 in Human
Calculated molecular weight
82.3 kDa
Background of EXT2
EXT2 encodes one of two glycosyltransferases involved in the chain elongation step of heparan sulfate biosynthesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02065-1 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of EXT2 Antibody (Center) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of lysate from T47D cell line,using EXT2 Antibody (Center).A02065-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with EXT2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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EXT2 Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-EXT2 Antibody (Center) (A02065-1)
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