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Product Info Summary
| SKU: | A02494 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-EZH1 Antibody
SKU/Catalog Number
A02494
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-EZH1 Antibody (Catalog # A02494). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
EZH1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-EZH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02494)
Host
Rabbit
Contents
EZH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
EZH1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EZH1. The immunogen is located within amino acids 180 - 230 of EZH1.
Cross-reactivity
Multiple isoforms of EZH1 are known to exist. EZH1 antibody is predicted to not cross-react with EZH2
Reactive Species
A02494 is reactive to EZH1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
85.3 kDa
Background of EZH1
EZH1 was initially identified as a homolog of the drosophila Enhancer of Zeste through transcription mapping of chromosome 17q21. Both EZH1 and the related protein EZH2 can form complexes with the noncanonical Polycomb repressive complex-2 (PRC2) and maintain repressive chromatin, but the PRC2-EZH1 complex mediates methylation of histone H3. Both EZH1 and EZH2 are thought to function in the maintenance of embryonic stem cell pluripotency and plasticity and recently have been shown to be essential for hair follicle homeostasis and wound repair.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02494 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
EZH1 antibody can be used for detection of EZH1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of EZH1 in mouse lung tissue lysate with EZH1 antibody at (A) 1 and (B) 2 μg/mL
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Immunohistochemistry of EZH1 in human lung tissue with EZH1 antibody at 5 μg/mL.
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Immunofluorescence of EZH1 in human lung tissue with EZH1 antibody at 20 μg/mL.
Specific Publications For Anti-EZH1 Antibody (A02494)
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