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Product Info Summary
| SKU: | M01750 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Ezrin Rabbit Monoclonal Antibody
SKU/Catalog Number
M01750
BM4996 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Ezrin Rabbit Monoclonal Antibody catalog # M01750. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ezrin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01750)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAAI-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Ezrin
Reactive Species
M01750 is reactive to EZR in Human
Observed Molecular Weight
80 kDa
Calculated molecular weight
69.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01750 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of Ezrin expression in HeLa cell lysate.
Specific Publications For Anti-Ezrin Rabbit Monoclonal Antibody (M01750)
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Customer Q&As
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4 Customer Q&As for Anti-Ezrin Rabbit Monoclonal Antibody
Question
Will anti-Ezrin Rabbit Monoclonal antibody M01750 work for WB with colon?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
According to the expression profile of colon, EZR is highly expressed in colon. So, it is likely that anti-Ezrin Rabbit Monoclonal antibody M01750 will work for WB with colon.
Boster Scientific Support
Answered: 2019-11-12
Question
Can you help my question with product M01750, anti-Ezrin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01750 anti-Ezrin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-11
Question
Is a blocking peptide available for product anti-Ezrin Rabbit Monoclonal antibody (M01750)?
T. Zhao
Verified customer
Asked: 2016-10-24
Answer
We do provide the blocking peptide for product anti-Ezrin Rabbit Monoclonal antibody (M01750). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-10-24
Question
We are currently using anti-Ezrin Rabbit Monoclonal antibody M01750 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
L. Kulkarni
Verified customer
Asked: 2014-09-12
Answer
The anti-Ezrin Rabbit Monoclonal antibody (M01750) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-09-12
