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Product Info Summary
| SKU: | A08075 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-F-BAR domain only protein 2 FCHO2 Antibody
SKU/Catalog Number
A08075
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-F-BAR domain only protein 2 FCHO2 Antibody (Catalog # A08075). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
FCHO2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-F-BAR domain only protein 2 FCHO2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08075)
Host
Rabbit
Contents
FCHO2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
FCHO2 antibody was raised against a 15 amino acid synthetic peptide near the center of human FCHO2. The immunogen is located within amino acids 340 - 390 of FCHO2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
FCHO2 antibody is predicted to not cross-react with other FCHO protein family members. At least the isoforms of FCHO2 are known to exist; this antibody will detect only the two longest isoforms.
Reactive Species
A08075 is reactive to FCHO2 in Human, Mouse, Rat
Applications
A08075 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
88.924kDa
Background of FCHO2
FCHO2 (FCH domain only 2) is a ubiquitously expressed member of the FCFBS superfamily characterized by FES-CIP4 homology (FCH) domain, an FBH domain, and an SH3 domain. Both FCHO2 and the related protein FCHO1 are mediators of clathrin-mediated endocytosis, acting to sculpt the initial bud site and recruit the clathrin machinery for clathrin-coated vesicle formation. FCHO2 binds to Eps15, an important adaptor protein in clathrin-mediated endocytosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FCHO2 antibody can be used for detection of FCHO2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FCHO2 in rat heart tissue lysate with FCHO2 antibody at (A) 1 and (B) 2 μg/ml
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Immunohistochemistry of FCHO2 in human heart tissue with FCHO2 antibody at 2.5 μg/mL.
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Immunofluorescence of FCHO2 in human heart tissue with FCHO2 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For FCHO2 (Source: Uniprot.org, NCBI)
Gene Name
FCHO2
Full Name
F-BAR domain only protein 2
Weight
88.924kDa
Superfamily
FCHO family
Alternative Names
FCH domain only 2 FCHO2 FCH and mu domain containing endocytic adaptor 2 F-BAR domain only protein 2|FCH domain only 2|FCH domain only protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FCHO2, check out the FCHO2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FCHO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-F-BAR domain only protein 2 FCHO2 Antibody (A08075)
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No publications found for A08075
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Customer Reviews
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