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Product Info Summary
| SKU: | A04372-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-FACL4/ACSL4 Antibody Picoband®
SKU/Catalog Number
A04372-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FACL4/ACSL4 Antibody Picoband® catalog # A04372-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-FACL4/ACSL4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04372-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FACL4/ACSL4 recombinant protein (Position: A36-E237).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04372-3 is reactive to ACSL4 in Human, Mouse, Rat
Observed Molecular Weight
79 kDa
Calculated molecular weight
79.2 kDa
Background of ACSL4
Long-chain-fatty-acid—CoA ligase 4 is an enzyme that in humans is encoded by the ACSL4 gene. It is mapped to Xq23. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04372-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human U20S whole cell, human PC-3 whole cell, human Hela whole cell, human Caco-2 whole cell, human HEL whole cell, human U-87MG whole cell, rat brain tissue, rat lung tissue, rat stomach tissue, rat PC-12 whole cell, mouse brain tissue, mouse lung tissue, mouse stomach tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (A04372-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: human HEL whole cell lysates,
Lane 6: human U-87MG whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: rat lung tissue lysates,
Lane 9: rat stomach tissue lysates,
Lane 10: rat PC-12 whole cell lysates,
Lane 11: mouse brain tissue lysates,
Lane 12: mouse lung tissue lysates,
Lane 13: mouse stomach tissue lysates,
Lane 14: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FACL4/ACSL4 antigen affinity purified polyclonal antibody (Catalog # A04372-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FACL4/ACSL4 at approximately 79 kDa. The expected band size for FACL4/ACSL4 is at 79 kDa.
Specific Publications For Anti-FACL4/ACSL4 Antibody Picoband® (A04372-3)
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